1. Pregnancy associates with alterations to the host and microbial proteome in vaginal mucosa
- Author
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Zuend, Christina Farr, Tobin, Nicole H, Vera, Trisha, Kotyrba, Lani, Noël‐Romas, Laura, Birse, Kenzie, Mutch, Sarah, Li, Fan, Lee, David, McCorrister, Stuart, Westmacott, Garrett, Aldrovandi, Grace M, and Burgener, Adam D
- Subjects
Reproductive Medicine ,Biomedical and Clinical Sciences ,HIV/AIDS ,Pediatric ,Pediatric Research Initiative ,2.1 Biological and endogenous factors ,Aetiology ,Infection ,Reproductive health and childbirth ,Adolescent ,Adult ,Energy Metabolism ,Female ,Humans ,Lactobacillus ,Mass Spectrometry ,Microbiota ,Middle Aged ,Mucous Membrane ,Pregnancy ,Proteome ,Vagina ,Young Adult ,HIV ,microbiome ,pregnancy ,proteomics - Abstract
ProblemPregnant women are at increased risk of HIV acquisition, but the biological mechanisms contributing to this observation are not well understood.Method of studyHere, we assessed host immune and microbiome differences in the vaginal mucosa of healthy pregnant and non-pregnant women using a metaproteomics approach. Cervicovaginal lavage (CVL) samples were collected from 23 pregnant and 25 non-pregnant women.ResultsMass spectrometry analysis of CVL identified 550 human proteins and 376 bacterial proteins from 11 genera. Host proteome analysis indicated 56 human proteins (10%) were differentially abundant (P
- Published
- 2020