1. An innovative intermittent hypoxia model for cell cultures allowing fast Po
- Author
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Mélanie, Minoves, Jessica, Morand, Frédéric, Perriot, Morgane, Chatard, Brigitte, Gonthier, Emeline, Lemarié, Jean-Baptiste, Menut, Jan, Polak, Jean-Louis, Pépin, Diane, Godin-Ribuot, and Anne, Briançon-Marjollet
- Subjects
Sleep Apnea, Obstructive ,Time Factors ,Cell Culture Techniques ,Endothelial Cells ,Breast Neoplasms ,Equipment Design ,Hypoxia-Inducible Factor 1, alpha Subunit ,Cell Hypoxia ,Culture Media ,Oxygen ,Blood-Brain Barrier ,Cell Line, Tumor ,Animals ,Humans ,Tumor Hypoxia ,Female ,Gases ,Hypoxia - Abstract
Performing hypoxia-reoxygenation cycles in cell culture with a cycle duration accurately reflecting what occurs in obstructive sleep apnea (OSA) patients is a difficult but crucial technical challenge. Our goal was to develop a novel device to expose multiple cell culture dishes to intermittent hypoxia (IH) cycles relevant to OSA with limited gas consumption. With gas flows as low as 200 ml/min, our combination of plate holders with gas-permeable cultureware generates rapid normoxia-hypoxia cycles. Cycles alternating 1 min at 20% O
- Published
- 2017