1. Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice.
- Author
-
Justice JP, Borchers MT, Lee JJ, Rowan WH, Shibata Y, and Van Scott MR
- Subjects
- Allergens immunology, Animals, Asthma immunology, Blood Proteins analysis, Bronchoalveolar Lavage Fluid chemistry, Bronchoalveolar Lavage Fluid immunology, Chemotaxis, Leukocyte immunology, DNA-Binding Proteins analysis, Eosinophil Granule Proteins, Eosinophils chemistry, Eosinophils cytology, GATA3 Transcription Factor, Gene Expression immunology, In Situ Hybridization, In Vitro Techniques, Interleukin-4 biosynthesis, Interleukin-5 biosynthesis, Male, Mice, Mice, Inbred C57BL, Plant Proteins immunology, RNA, Messenger analysis, Th2 Cells immunology, Trans-Activators analysis, Transcription, Genetic immunology, DNA-Binding Proteins genetics, Eosinophils immunology, Interleukin-4 analysis, Interleukin-5 analysis, Respiratory Hypersensitivity immunology, Ribonucleases, Trans-Activators genetics
- Abstract
Allergen-induced recruitment of T lymphocytes and eosinophils to the airways is associated with increased expression of the transcription factor GATA-3. In this study, the relationship between airway inflammation and GATA-3 expression in the lungs was investigated using ragweed-sensitized C57BL/6J mice. Intratracheal ragweed challenge increased both the number of GATA-3-expressing cells in the perivascular and peribronchial regions and the amount of expression per cell. Interleukin (IL)-4 and IL-5 levels in bronchoalveolar lavage fluid were upregulated in parallel with GATA-3 expression. GATA-3 mRNA and protein colocalized to eosinophils. Eosinophils isolated from the lungs and stimulated with phorbol 12-myristate 13-acetate and/or A-23187 released IL-5. The release was inhibited by actinomycin D, which indicates that de novo synthesis of the cytokine was involved. Western blot analysis of proteins from isolated eosinophils demonstrated expression of the p50 subunit of nuclear factor-kappaB, a transcription factor that is implicated in control of GATA-3 expression. These data provide evidence that allergen challenge increases GATA-3 and proinflammatory cytokine expression by pulmonary eosinophils, which could provide positive feedback for the inflammatory response.
- Published
- 2002
- Full Text
- View/download PDF