1. Resistin deficiency in mice has no effect on pulmonary responses induced by acute ozone exposure.
- Author
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Razvi SS, Richards JB, Malik F, Cromar KR, Price RE, Bell CS, Weng T, Atkins CL, Spencer CY, Cockerill KJ, Alexander AL, Blackburn MR, Alcorn JL, Haque IU, and Johnston RA
- Subjects
- Airway Resistance drug effects, Animals, Bronchoconstrictor Agents pharmacology, Female, Lung drug effects, Lung metabolism, Lung pathology, Male, Methacholine Chloride pharmacology, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Pneumonia chemically induced, Resistin blood, Air Pollutants toxicity, Ozone toxicity, Pneumonia metabolism, Resistin genetics
- Abstract
Acute exposure to ozone (O3), an air pollutant, causes pulmonary inflammation, airway epithelial desquamation, and airway hyperresponsiveness (AHR). Pro-inflammatory cytokines-including IL-6 and ligands of chemokine (C-X-C motif) receptor 2 [keratinocyte chemoattractant (KC) and macrophage inflammatory protein (MIP)-2], TNF receptor 1 and 2 (TNF), and type I IL-1 receptor (IL-1α and IL-1β)-promote these sequelae. Human resistin, a pleiotropic hormone and cytokine, induces expression of IL-1α, IL-1β, IL-6, IL-8 (the human ortholog of murine KC and MIP-2), and TNF. Functional differences exist between human and murine resistin; yet given the aforementioned observations, we hypothesized that murine resistin promotes O3-induced lung pathology by inducing expression of the same inflammatory cytokines as human resistin. Consequently, we examined indexes of O3-induced lung pathology in wild-type and resistin-deficient mice following acute exposure to either filtered room air or O3. In wild-type mice, O3 increased bronchoalveolar lavage fluid (BALF) resistin. Furthermore, O3 increased lung tissue or BALF IL-1α, IL-6, KC, TNF, macrophages, neutrophils, and epithelial cells in wild-type and resistin-deficient mice. With the exception of KC, which was significantly greater in resistin-deficient compared with wild-type mice, no genotype-related differences in the other indexes existed following O3 exposure. O3 caused AHR to acetyl-β-methylcholine chloride (methacholine) in wild-type and resistin-deficient mice. However, genotype-related differences in airway responsiveness to methacholine were nonexistent subsequent to O3 exposure. Taken together, these data demonstrate that murine resistin is increased in the lungs of wild-type mice following acute O3 exposure but does not promote O3-induced lung pathology., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
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