1. Immunotherapy of human cervical high-grade cervical intraepithelial neoplasia with microparticle-delivered human papillomavirus 16 E7 plasmid DNA.
- Author
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Sheets EE, Urban RG, Crum CP, Hedley ML, Politch JA, Gold MA, Muderspach LI, Cole GA, and Crowley-Nowick PA
- Subjects
- Adult, Antibodies, Viral immunology, Antibody Specificity, Capsules, DNA-Binding Proteins immunology, Dose-Response Relationship, Drug, Electrosurgery, Female, Humans, Oncogene Proteins, Viral immunology, Papillomaviridae, Papillomavirus E7 Proteins, Particle Size, Plasmids, Plasminogen, T-Lymphocytes immunology, Treatment Outcome, Uterine Cervical Neoplasms immunology, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms surgery, Virion, Uterine Cervical Dysplasia immunology, Uterine Cervical Dysplasia pathology, Uterine Cervical Dysplasia surgery, Antigens, Viral genetics, DNA, Viral administration & dosage, Immunotherapy methods, Oncogene Proteins, Viral genetics, Uterine Cervical Neoplasms therapy, Uterine Cervical Dysplasia therapy
- Abstract
Objective: The purpose of this study was to evaluate the safety of the administration of a bacterial expression plasmid encoding a 13 amino acid sequence that is highly homologous with human papillomavirus E7 within poly (lactide-co-glycolide) microparticles (ZYC101) in women with HLA A2+ antigen and persistent cervical intraepithelial neoplasia grade 2/3 and human papillomavirus 16., Study Design: Fifteen women entered an institutional review board-approved dose-escalating phase I study with the use of three levels of blood monitoring and urine studies, Papanicolaou tests, and colposcopy. Escalation required no serious adverse events. Immunologic responses were evaluated in peripheral blood with the use of human papillomavirus peptide-stimulated interferon gamma enzyme-linked immunosorbent assay for T-cell reactivity. In cervical secretions, immunoglobulin A anti-human papillomavirus 16 E2 concentrations were measured. Three doses every 3 weeks were followed 4 weeks later by surgical excision., Results: No serious adverse events occurred. Five women had complete histologic responses; 11 women had human papillomavirus-specific T-cell responses. Four of five complete histologic responses developed immunoglobulin A anti-E2-specific antibody., Conclusion: ZYC101 warrants further investigation because of a 33% complete histologic responses, a 73% immunologic response, and no serious adverse events.
- Published
- 2003
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