1. Characterization of the gut microbiota of Nicaraguan children in a water insecure context
- Author
-
Seungjun Lee, Warren M. Wilson, Jiyoung Lee, Pallavi Oruganti, Rebecca Garabed, Adelaide Stull Cary, Alba C. Mayta Apaza, Samuel Vilchez, and Barbara A. Piperata
- Subjects
Rural Population ,Salmonella ,Nicaragua ,Pilot Projects ,Context (language use) ,Biology ,Gut flora ,medicine.disease_cause ,Food Supply ,fluids and secretions ,Water Quality ,Environmental health ,Genetics ,medicine ,Humans ,Child ,Ecology, Evolution, Behavior and Systematics ,Feces ,Campylobacter ,Infant, Newborn ,Infant ,Delivery mode ,biology.organism_classification ,Gut microbiome ,Gastrointestinal Microbiome ,Microbial population biology ,Child, Preschool ,Anthropology ,Anatomy - Abstract
OBJECTIVES The gut microbiota varies across human populations. The first years of life are a critical period in its development. While delivery mode and diet contribute to observed variation, the additional contribution of specific environmental factors remains poorly understood. One factor is waterborne enteric pathogen exposure. In this pilot study, we explore the relationship between household water security and the gut microbiota of children. METHODS From Nicaraguan households (n = 39), we collected drinking water samples, as well as fecal samples from children aged one month to 5.99 years (n = 53). We tested water samples for total coliforms (CFU/mL) and the presence of common enteric pathogens. Composition and diversity of the gut microbiota were characterized by 16S rRNA sequencing. Households were classified as having drinking water that was "low" (
- Published
- 2019
- Full Text
- View/download PDF