1. Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer.
- Author
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Levink IJM, Nesteruk K, Visser DI, Sieuwerts AM, Fernandes CJC, Jansen MPHM, van Driel LMJW, Poley JW, Peppelenbosch MP, Cahen DL, Fuhler GM, and Bruno MJ
- Subjects
- Carcinoma, Pancreatic Ductal metabolism, Early Detection of Cancer methods, Endosonography, Humans, Pancreatic Neoplasms metabolism, Prospective Studies, Biomarkers metabolism, Carcinoma, Pancreatic Ductal diagnosis, Pancreatic Juice metabolism, Pancreatic Neoplasms diagnosis
- Abstract
Introduction: Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers., Methods: Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0-4 vs 4-8 vs 8-15 minutes)., Results: Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible., Discussion: The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.
- Published
- 2020
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