1. Aqueous Extract of Mosla chinensis Inhibits Mast Cell-Mediated Allergic Inflammation.
- Author
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Kim, Hui-Hun, Yoo, Jin-Su, Shin, Tae-Yong, and Kim, Sang-Hyun
- Subjects
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ALLERGY drug therapy , *ANAPHYLAXIS , *ANIMAL experimentation , *BIOLOGICAL assay , *CELL culture , *DOSE-effect relationship in pharmacology , *ENZYME-linked immunosorbent assay , *GENE expression , *HISTAMINE , *INFLAMMATION , *INTERLEUKINS , *MAST cells , *MICE , *POLYMERASE chain reaction , *RESEARCH funding , *STATISTICS , *TUMOR necrosis factors , *WESTERN immunoblotting , *PLANT extracts , *DATA analysis , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Allergic inflammatory diseases such as food allergy, asthma, sinusitis, and atopic dermatitis are increasing worldwide. In this study, we investigated the effects of aqueous extract of Mosla chinensis Max. (AMC) on mast cell-mediated allergic inflammation and studied the possible mechanism of this action. AMC inhibited compound 48/80-induced systemic and immunoglobulin E (IgE)-mediated local anaphylaxis. AMC reduced intracellular calcium levels and downstream histamine release from rat peritoneal mast cells activated by compound 48/80 or IgE. In addition, AMC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in human mast cells. The inhibitory effect of AMC on cytokine expression was nuclear factor (NF)-κB dependent. Our results indicate that AMC inhibits mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of proinflammatory cytokines and the involvement of calcium and NF-κB in these effects. AMC might be a possible therapeutic candidate for allergic inflammatory disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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