1. Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae)
- Author
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Fang-yun Cheng, Qi-xiang Zhang, and Lin Li
- Subjects
Genetics ,Germplasm ,Paeonia lactiflora ,Polymorphism, Genetic ,DNA, Plant ,Genotype ,food and beverages ,Plant Science ,Biology ,Herbaceous plant ,Paeonia ,biology.organism_classification ,Sequence repeat ,Genetic Loci ,Microsatellite ,Genomic library ,Alleles ,Genome, Plant ,Ecology, Evolution, Behavior and Systematics ,DNA Primers ,Microsatellite Repeats ,Tetranucleotide Repeats - Abstract
Premise of the study : Microsatellite (simple sequence repeat, SSR) primers were developed for the Chinese peony, P. lactifl ora , to investigate the diversity within Chinese peony germplasm resources. Methods and Results : Using an SSR-enriched genomic library, a set of 10 unique polymorphic genomic SSRs was developed and characterized. The primers amplifi ed 61 alleles in all 10 loci, including di-, tri-, and tetranucleotide repeats. The primers were also effective for P. veitchii and P. obovata . Conclusions : The new primers will be useful for genetic research of the Chinese peony and extend the repertoire of SSR markers available to study the herbaceous taxa in Paeonia .
- Published
- 2011
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