1. Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite
- Author
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Mohamed H. Madkour, Alexander Steinbüchel, Daniel Heinrich, Mansour A. Alghamdi, and Ibraheem I. Shabbaj
- Subjects
Ralstonia eutropha ,chemistry.chemical_classification ,Downstream process ,Chromatography ,biology ,Polyhydroxyalkanoates ,Extraction (chemistry) ,Biophysics ,Poly-3-hydroxybutyrate ,Polymer ,biology.organism_classification ,Applied Microbiology and Biotechnology ,PHA recovery ,Microbiology ,Sodium hypochlorite ,chemistry.chemical_compound ,Volume (thermodynamics) ,chemistry ,Ralstonia ,Poly (3-hydroxybutyrate) ,Original Article ,Eutropha - Abstract
Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure product at low costs. This study presents a simplified method for large scale poly(3-hydroxybutyrate), poly(3HB), extraction using sodium hypochlorite. Poly(3HB) was extracted from cells of Ralstonia eutropha H16 at almost 96% purity. At different extraction volumes, a maximum recovery rate of 91.32% was obtained. At the largest extraction volume of 50 L, poly(3HB) with an average purity of 93.32% ± 4.62% was extracted with a maximum recovery of 87.03% of the initial poly(3HB) content. This process is easy to handle and requires less efforts than previously described processes.
- Published
- 2012
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