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Background Epidermal Langerhans cells ( LC) expressing the high-affinity receptor for IgE (Fcε RI) play a key role in atopic dermatitis ( AD). AD skin is highly colonized with Staphylococcus aureus (S.a.), which are sensed by Toll-like receptor 2 ( TLR2). We hypothesized that TLR2 may impact on the expression of Fcε RI on LC. Objectives To study a putative impact of TLR2 signaling on Fcε RI, we analyzed Fcε RI and known transcription factors of the receptor after ligand binding to TLR2. Methods We generated LC from CD34+ progenitors in vitro ( CD34 LC) expressing Fcε RI and TLR2 as well as its partners TLR1 and TLR6. The expression of Fcε RI and known transcription factors of the receptor was analyzed on the protein and RNA level by flow cytometry, Western blotting, and real-time PCR. Results For CD34LC from 123 donors, we observed a high heterogeneity in FcεRI surface expression correlating with mRNA level of its α-chain. Stimulation of TLR1/2 or TLR2/6 dramatically down-regulated FcεRI on protein and mRNA level of both α- and γ-chain. Further analysis of putative transcription factors for FCER1A revealed the lack of GATA1 in CD34LC, weak expression of ELF1 and YY1, and high expression of PU.1. While ELF1 and YY1 appeared to be little affected by TLR2 engagement, PU.1 was significantly down-regulated. Conclusions Taken together, our findings show that in human, LC ligation of TLR2 by S.a.-derived products down-regulates Fcε RI and its transcription factor PU.1, thus suggesting that Fcε RI is controlled by PU.1 in these cells. [ABSTRACT FROM AUTHOR]