1. Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein).
- Author
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Mori Y, Okazaki F, Inuo C, Yamaguchi Y, Masuda S, Sugiura S, Fukuie T, Nagao M, Tsuge I, Yosikawa T, Yagami A, Matsunaga K, Fujisawa T, Ito K, Narita H, and Kondo Y
- Subjects
- Adolescent, Adult, Antigens, Plant administration & dosage, Antigens, Plant adverse effects, Carrier Proteins administration & dosage, Carrier Proteins adverse effects, Carrier Proteins immunology, Child, Enzyme-Linked Immunosorbent Assay, Female, Food Hypersensitivity blood, Food Hypersensitivity immunology, Gibberellins administration & dosage, Gibberellins adverse effects, Gibberellins immunology, Humans, Male, Middle Aged, Recombinant Proteins administration & dosage, Recombinant Proteins adverse effects, Recombinant Proteins immunology, Young Adult, Antigens, Plant immunology, Food Hypersensitivity diagnosis, Immunoglobulin E blood, Prunus persica adverse effects
- Abstract
Background: Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy., Methods: Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7)., Results: Peach-allergic patients (n=27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n=17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P<0.05)., Conclusions: We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction., (Copyright © 2018 SEICAP. Published by Elsevier España, S.L.U. All rights reserved.)
- Published
- 2018
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