1. Raw dark fermentation effluent to support heterotrophic microalgae growth: microalgae successfully outcompete bacteria for acetate
- Author
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A. Fayet, Violette Turon, Jean-Philippe Steyer, Eric Fouilland, Eric Trably, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), IDEALG ANR-10-BTBR-04, ANR-10-BTBR-0004,IDEALG,Biotechnologies pour la valorisation des macroalgues(2010), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
- Subjects
2. Zero hunger ,Chlorella sorokiniana ,Heterotrophic cultivation ,[SDE.MCG]Environmental Sciences/Global Changes ,Heterotroph ,Volatile fatty acids (VFAs) ,Biomass ,Dark fermentation ,Biology ,biology.organism_classification ,complex mixtures ,6. Clean water ,[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems ,Bacterial diversity ,Yield (chemistry) ,Botany ,Biohydrogen ,Food science ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Agronomy and Crop Science ,Effluent ,Bacteria - Abstract
International audience; Coupling dark fermentation (DF), which produces hydrogen from diverse effluents or solid waste, and heterotrophic cultivation of microalgae, which produces lipids, carbohydrates and proteins, is a promising and innovative solution for developing sustainable biorefineries. The use of a raw DF effluent, containing acetate and butyrate, to support the heterotrophic growth of Chlorella sorokiniana was investigated. All the acetate in sterilized and unsterilized DF effluent was exhausted in less than three days of heterotrophic cultivation, whereas butyrate was not used by the microalgae. The microalgae biomass reached 0.33 g L− 1 with a carbon yield on acetate of 55%. The algal yield was higher than previously reported for synthetic DF effluent. It was concluded that compounds other than volatile fatty acids were present in the DF effluent and these could be consumed by the microalgae. After the acetate had been exhausted, butyrate was consumed by facultative and strict aerobic bacteria originating from the DF effluent. The concentration of the bacterial community increased during the experiment but did not have any significant impact on heterotrophic microalgae growth. A high microalgal biomass yield was achieved without requiring the DF effluent to be sterilized.
- Published
- 2015
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