1. Novel graphene-oxide-coated SPR interfaces for biosensing applications
- Author
-
Aleksey V. Arsenin, Yu V. Stebunov, Valentyn S. Volkov, D. Yu. Fedyanin, and Dmitry I. Yakubovsky
- Subjects
Materials science ,Graphene ,Biological objects ,Oxide ,chemistry.chemical_element ,Nanotechnology ,Small molecule ,law.invention ,Nanomaterials ,chemistry.chemical_compound ,chemistry ,law ,Surface plasmon resonance ,Carbon ,Biosensor - Abstract
Carbon allotropes-based nanomaterials possess unique physical and chemical properties including high surface area, the possibility of pi-stacking interaction with a wide range of biological objects, rich availability of oxygen-containing functional groups in graphene-oxide (GO), and excellent optical properties, which make them an ideal candidate for use as a universal immobilization platform in SPR biosensing. Here, we propose a new surface plasmon resonance (SPR) biosensing interface for sensitive and selective detection of small molecules. This interface is based on the GO linking layers deposited on the gold/copper surface of SPR sensor chips. To estimate the binding capacity of GO layers, modification of carboxyl groups to N-Hydroxysuccinimide esters was performed in the flow cell of SPR instrument. For comparison, the same procedure was applied to commercial sensor chips based on linking layers of carboxymethylated dextran.
- Published
- 2017