1. Variation in cell-associated unspliced HIV RNA on antiretroviral therapy is associated with the circadian regulator brain-and-muscle-ARNT-like-1
- Author
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Chang, Christina C, Naranbhai, Vivek, Stern, Jared, Roche, Michael, Dantanarayana, Ashanti, Ke, Ruian, Tennakoon, Surekha, Solomon, Ajantha, Hoh, Rebecca, Hartogensis, Wendy, Hecht, Frederick M, Sikaris, Ken, Price, David J, Elliott, Julian H, Deeks, Steven G, Churchill, Melissa, Cameron, Paul U, Hengartner, Nicolas, Perelson, Alan S, and Lewin, Sharon R
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Infectious Diseases ,Sexually Transmitted Infections ,Genetics ,Neurosciences ,HIV/AIDS ,ARNTL Transcription Factors ,Anti-Retroviral Agents ,Blood Cells ,Cells ,Cultured ,Female ,Gene Expression Profiling ,HIV ,HIV Infections ,Host-Pathogen Interactions ,Humans ,Male ,Middle Aged ,RNA ,Viral ,Real-Time Polymerase Chain Reaction ,Transcription ,Genetic ,brain-and-muscle-ARNT-like-1 ,circadian rhythm ,HIV latency ,HIV transcription ,stress ,unspliced RNA ,Biological Sciences ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Virology ,Biomedical and clinical sciences ,Health sciences - Abstract
Objective(s)To determine whether variation in cell-associated unspliced (CA-US) HIV RNA in HIV-infected individuals on antiretroviral therapy (ART) has a circadian basis.MethodsProspective observational study of HIV-infected individuals on ART. Blood was collected on three occasions and CA-US HIV RNA and mRNA of the circadian-locomotor-output-cycles-kaput (CLOCK)-associated genes quantified by real time PCR. CLOCK-associated proteins were over-expressed in a cell line stably transfected with an HIV long-terminal repeat (LTR) luciferase reporter.ResultsUsing a mixed effects model, there was a significant increase in log-CA-US RNA at the third visit compared with the first visit (effect size of 0.619 with standard error (SE) of 0.098, P
- Published
- 2018