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Purpose: This study was to compare the effectiveness of chiropractic manipulation and mulligan mobilization on pain and functionality in health care workers diagnosed with chronic low back pain. Material and Methods: Forty volunteers aged 20-50 years (Mulligan group (MMG) 27.70±6.57, chiropractic group (CMG) 31.15± 8.15) who were diagnosed with chronic low back pain were included in the study. All cases were randomly divided into two groups as CMG (n:20; 11 females, 9 males) and MMG (n:20; 17 females, 3 males). All treatment groups were evaluated with a numerical pain scale, Oswestry questionnaire, and goniometer. Chiropractic manipulation was applied to CMG and mulligan mobilization was applied to MMG twice a week for 4 weeks. No extra application has been made other than these treatments. Results: Evaluation was done before and after treatment. There is no difference between groups in terms of anthropometrics. (p>0.05). In-group analysis results showed significant differences in all evaluation parameters in both groups and showed a positive improvement (p0.05). Conclusion: There was no superiority between chiropractic manipulation and mulligan mobilization techniques in terms of successful results in the treatment of chronic low back pain. The use of both methods according to appropriate conditions is accepted in patients with chronic low back pain.