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Wheat sharp eyespot is a prevalent soil-borne disease that causes substantial economic losses in agriculture. Metconazole, a new triazole broad-spectrum fungicide, has demonstrated effective control of soil-borne diseases. Multi-walled carbon nanotubes (MWCNTs) are an innovative adsorbent material known for their large surface area and high absorptive capacity. This study identifies MWCNTs as the optimal adsorption material for metconazole, achieving an adsorption rate of 85.27% under optimal conditions (stirring time of 30 min and feeding ratio of 6:1). The optimized formula consists of 1.5% dispersant sodium wood, 1% emulsifier BY-112, 2% AEO-15, 3% glycol, 3% filmogen, and 4% red dyes. A 0.5% MWCNT–metconazole suspension concentrate for seed coating (FSC) significantly enhances the inhibitory effect of metconazole on wheat growth and promotes root development. At the tillering stage, a coating ratio of 1:100 shows a marked impact on wheat growth, and MWCNTs can improve the control effect of metconazole to Rhizoctonia cerealis. This work offers a novel approach for applying metconazole in a wheat suspension concentrate for seed coating. [ABSTRACT FROM AUTHOR]

TFC (10% thifluzamide–fludioxonil–clothianidin) is a novel wheat seed-coating agent. In the field, we confirmed that 10% TFC plays a positive role in preventing soil-borne diseases and promoting wheat seedling growth. However, its effects on rhizosphere microecology and the underlying molecular mechanism are not fully understood. Field trials revealed a positive effect on the biomass, plant height, and root length of wheat sharp eyespots in a Yingshang field, with 95.3% control efficiency. The effects of 10% TFC on the rhizosphere soil microbiome of young wheat plants were evaluated using high throughput sequencing technology. The results demonstrated that seed-coating agents significantly changed bacterial and fungal communities, and reduced the number of bacteria but increased the number of fungi. Sequence analysis revealed that the abundance of Proteobacteria, Actinobacteria, and Patescibacteria in bacteria and Ascomycota, Mortierellomycota, and Basidiomycota in fungi were significantly enriched, which have been reported as being beneficial for plant growth and pathogen resistance. In contrast, the abundance of Mucoromycota in fungi was reduced, and most of the related genera identified were pathogenic to plants. In this study, 15-day-old wheat plant tissues treated with 10% TFC were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of 10% TFC on seedling growth. The comparative analysis of Triticum aestivum L. libraries identified 8286 differentially expressed genes (DEGs), of which 2290 and 5996 genes were up- and downregulated in seedling growth in the presence of 10% TFC, respectively. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses were performed for up- and downregulated DEGs separately, showing that these DEGs were enriched for terms related to the phenylpropanoid biosynthesis pathway, the protein products of which promote cell differentiation and seedling growth. This research provides comprehensive insights into its effects on wheat seedling growth and the rhizosphere microecology of seed coatings and provides important insights into their regulation and into understanding the potential benefits of seed coatings in disease management and plant growth promotion. [ABSTRACT FROM AUTHOR]

Seed vigor is a crucial indicator comprehensively assessing the quality of seeds, reflecting the growth advantage and production potential of seeds, and has a significant effect on seeds' stress resistance. Identifying and controlling loci related to wheat seed vigor is essential for accelerating genetic trait gains. Here, we performed a large genome-wide association study (GWAS) to identify several significant quantitative trait loci (QTLs) associated with seed vigor-related traits. A total of 404 wheat samples with diverse genetic backgrounds were used as experimental materials. Twenty-eight loci significantly associated with seed vigor-related traits in wheat were identified, distributed on chromosomes 3A, 4A, 5B, 7A, and 7B. Two potential novel loci controlling wheat seed vigor were discovered, with a total of 80 candidate genes associated with seed vigor located on these loci. Among them, TraesCS4A01G020000.1 encodes a late embryogenesis abundant (LEA) protein gene, and TraesCS5B01G298500.1 encodes a helicase gene, both showing specific expression in seeds and highly correlated with seed vigor. Overall, these findings provide valuable insights for the future application of these genes in wheat breeding. [ABSTRACT FROM AUTHOR]

Prior to dispatch from manufacturing facilities, seeders require rigorous performance evaluations for their seeding capabilities. Conventional manual inspection methods are notably less efficient. This study introduces a wheat seeding detection approach anchored in an enhanced YOLOv5s image-processing technique. Building upon the YOLOv5s framework, we integrated four CBAM attention mechanism modules into its model. Furthermore, the traditional upsampling technique in the neck layer was superseded by the CARAFE upsampling method. The augmented model achieved an mAP of 97.14%, illustrating its ability to elevate both the recognition precision and processing speed for wheat seeds while ensuring that the model remains lightweight. Leveraging this advanced model, we can effectively count and locate seed images, enabling the precise calculation and assessment of sowing uniformity, accuracy, and dispersion. We established a sowing test bench and conducted experiments to validate our model. The results showed that after the model was improved, the average accuracy of wheat recognition was above 97.55% under different sowing rates and travel speeds. This indicates that this method has high precision for the total number of seed particles. The sowing rate and sowing travel speed were consistent with manual measurements and did not significantly affect uniformity, accuracy, or dispersion. [ABSTRACT FROM AUTHOR]

Seeds stored for a prolonged period are subject to aging and a reduction in germination potential (GP), which will negatively affect seed sales. Rare-earth elements have a synergistic effect on the improvement of seed GP. In this study, we examined the effects of neodymium on biochemical components, the antioxidant protective system, and metabolism-related enzymes during germination of naturally and artificially aged seeds of three wheat cultivars. Seed germination indices, biochemical substance contents, and enzyme activities decreased after seed aging. Soaking seeds in a neodymium nitrate solution revived aged wheat seeds at an optimal concentration of 20 µmol/L for 8 h. Soaking in a neodymium nitrate solution increased the GP4 (by 2.25–60.9%), germination index (by 1.69–29.2%), and vigor index (by 3.36–18.7%) of aged seeds. Compared with non-soaked seeds, soaking significantly changed the contents of biochemical substances, and the activities of antioxidant protective enzymes and metabolic enzymes in seedlings were increased. Soaking with neodymium may revive aged seeds by regulating the synthesis of soluble sugars, soluble proteins, chlorophyll, and carotenoids and decomposing malondialdehyde in the germinating seed. Root dehydrogenase and amylase showed different responses to the aging modes. The differential responses of root dehydrogenase and amylase may reflect differences in the resistance of enzymes to long-term mild seed aging and short-term severe environmental aging. [ABSTRACT FROM AUTHOR]

Delayed sowing of wheat is a common problem in Punjab that exacerbates serious yield loss. However, individual or combined applications of seed rate and slow-release nitrogen fertilizer significantly impacted their efficacy in improving crop growth and productivity. In this regard, the present study explored the potential of slow-release nitrogen fertilizers (control (CK), simple urea (SU), neem-coated urea (NCU), and sulfur-coated urea (SCU)) to improve the growth, yield, and physiological and biochemical attributes of delayed sown wheat with three seed rate [(100 kg ha−1 (S1), 125 kg ha−1 (S2), and 150 kg ha−1 (S3)]. The study was carried out at the Postgraduate Agricultural Research Station of the University of Agriculture Faisalabad in 2018–19 and 2019–20. The study findings revealed that the sulfur-coated urea performed better under S3 seed rate. Combining SCU with S3 significantly increased leaf area index by 0.99 cm2 and plant height by 8.24% compared to simple urea, maximum spikelets, and spike length by 3.9 and 3.8 cm, respectively. The SCU with S3 treatment also increased the biological yield by 43% and 41% and the grain yield by 46% in 2018–2019 and 2019–2020, respectively, compared to CK. Similarly, higher N contents in straw and grain were recorded in the interaction of SCU with S3 treatment. Likewise, the SCU with S3 treatment enhanced the physiological attributes, i.e., chlorophyll contents and transpiration rate, by 18% and 25%, respectively, and decreased internal CO2 by 25.5%, compared to CK. In conclusion, applying sulfur-coated urea with a seed rate of 150 kg ha−1 could be considered a potential strategy for improving the growth and productivity of delayed sown wheat. [ABSTRACT FROM AUTHOR]

The present work aimed to test selected microalgae strains from the Mosonmagyaróvár Algae Culture Collection (MACC) on germination ability and certain physiological processes in winter wheat (Triticum aestivum L.) plants. Germination tests showed substantial differences between the strains, meaning that certain strains (such as MACC-430, MACC-612, MACC-922) improved the germination processes while others performed worse (MACC-438, MACC-755) than the control in a concentration-dependent manner. The germination index of seeds treated with MACC-430 @ 1 g L−1 concentrations was 87, while that of the control was 45. The mungbean rooting bioassay proves that microalgae biomass may exhibit auxin-like activity, especially in strain MACC-612 (Nostoc sp.), which was characterized by the highest endogenous level of plant growth regulator indole-3-acetic-acid among the selected strains. Foliar spray on the leaves of developed plants did not significantly alter the photosynthetic processes, but it influenced the secondary metabolite composition. After the application of microalgae biomass, there were also changes in plant hormones, including salicylic acid, abscisic acid, and jasmonic acid-leucine/isoleucine conjugate compositions, which play a role in plant stress signaling in plants. A decrease in indole-3-acetic acid was also observed in the Mv Nádor cultivar. These results suggest that the application of certain microalgae strains can be used effectively to improve the germination of wheat seeds, and as a foliar spray, they may also modify the acclimation processes in a genotype-dependent way. [ABSTRACT FROM AUTHOR]

In this study, the allelochemical-induced effects of the leaf water extract of hemp (Cannabis sativa) on seed germination and seedling growth were investigated in durum wheat (Triticum durum) cv. Mongibello and barley (Hordeum vulgare) cv. Alamo. The seeds were imbibed in 25%, 50%, 75%, or 100% solution concentrations, obtained from a 2% hemp leaf extract, and they were germinated at 20 and 10 °C. The 2% extract stock solution (i.e., 100% solution concentration) was obtained by extracting 20 g of leaf powder in 1 L distilled water (2%, w/v). A control of seeds imbibed in distilled water was considered. Germination was scored daily and at the end of the germination test, the final germination percentage (FGP), mean germination time (MGT), germination index (GI), and vigor index (VI) were calculated. The radicles and shoots of the germinated seeds of both species were also measured for their length and weight. The analyses revealed the presence of phenols in the hemp leaf extract, which were the highest in the 100% concentration (13.14 ± 0.20 mg gallic acid equivalent-GAE g−1 extract). At 20 °C, increasing the concentration had either minor (in wheat) or no effects (in barley) on the seed germination. At 10 °C, the germination was reduced by the increasing concentration, suggesting a potential allelopathic effect of the extract. However, at 100% concentration, the seeds germinated significantly better that those at 75% concentration. At 20 °C, increasing concentration progressively depressed both the radicle and shoot elongation in both species. At 10 °C, the radicle elongation was depressed by the extract, irrespective of the concentration, whilst the shoots were as long as those of the control in wheat, and smaller at the 50 and 75% concentrations in barley. We can conclude that the water extract of hemp leaves can have a negative impact on wheat and barley seed germination and seedling growth, particularly at temperatures below optimal. However, the high concentration of phenols in the extract may stimulate seed germination and seedling growth. Thus, in semi-arid areas, such as those of the Mediterranean regions, sowings of wheat and barley after hemp at an optimal (i.e., 20 °C) or nearly optimal temperature are recommended in order to minimize the combined stressing effects of the temperature and allelopathic leaf extract upon plant establishment, which may limit a successful crop establishment. [ABSTRACT FROM AUTHOR]

Through allelopathic interactions, plants may either suppress competing species or promote those that may help them better adapt to their environment. The purpose of this research was to determine how the root exudates of 15 common weeds affected the germination of wheat (Triticum aestivum L.). Every other day, 15 seeds were dispersed over Petri dishes (with filter paper) and pots (with garden soil) and treated with 1 mL and 5 mL of aqueous root exudates, respectively. Distilled water had the highest germination rate at 86.33% and the lowest at 64.00% (p = 0.001) for Commelina benghalensis in the Petri dish containing root exudates. The seed germination percentage of the pot condition was the lowest, at 68.45% (p = 0.004), for Solanum nigrum, while the control was 87.23%. Similarly, the lowest shoot length, 12.01 cm (p = 0.0025) in Mikania micrantha, and the lowest root length of 2.17 cm (p = 0.0048) in Leucas aspera, were recorded, whereas the control was 19.13 cm and 3.46 cm, respectively, in Petri dishes. In addition, the lowest shoot and root growth were 9.72 cm (p = 0.0004) in Mikania micrantha and 4.34 cm (p = 0.0019) in Spilanthes acmella, while the control was at 20.13 cm and 6.42 cm, respectively, for pot culture. Furthermore, in seedlings, biomass studies of treated T. aestivum showed elevated malonaldehyde (MDA) levels in both Petri dishes and pot cultures. However, chlorophyll a and b levels, as well as those of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), are lower than in the control group. Both enzymes were elevated in pot cultures compared to those grown in Petri plates. The dry weight of T. aestivum seedlings cultivated in pots and Petri dishes demonstrated its considerable allelopathic influence. This research found that the root exudates of all the weeds studied could have the capacity to impede wheat seed germination as well as the development of wheat seedlings. These inhibitory effects were higher in Petri dishes than in pot cultures. [ABSTRACT FROM AUTHOR]

Crop seeding depth is an important parameter in agrotechnologies, but how can seeding depth automatically be maintained in on-farm soil conditions with different textures, granulometric compositions, structural contents and penetration resistances? For this reason, an on-farm field experiment was conducted in the Panevėžys district (Lithuania) during 2020–2022. The field was divided into five zones (EZ1–EZ5) according to soil electrical conductivity. In addition, uniform and variable seeding depths were compared. The results of the investigations showed that soil electrical conductivity was highly correlated with sand (r = −0.867; p ≤ 0.010 > 0.001) and silt (r = 0.871; p ≤ 0.010 > 0.001) contents. The seeding method mainly did not have a significant effect on soil physical properties and winter wheat germination, development and productivity. Higher differences were observed among field zones. The winter wheat seeding depth varied from 27.74 to 33.12 mm between the two most different soil electrical conductivity zones. In zones with variable seeding depths, winter wheat seeds sprouted the most abundantly, and germination reached 99% (in EZ3 and EZ4). In EZ1, EZ2 and EZ4, the yields of grain were the highest and were significantly higher than that in the loamy sand of EZ5. The 1000-grain mass was not affected by any of the tested factors. The results suggest the need for further research in fields with a wider range of soil electrical conductivity. This can increase the variation in seeding depth and reveal interactions among the factors in more detail. [ABSTRACT FROM AUTHOR]

The continual re-evaluation of agronomic practices is necessary to improve crop performance and sustainability of the production of winter wheat (Triticum aestivum L.), particularly as genetics and climate conditions change. Recommendations made about winter wheat planting dates, spacing, variety, and seed rates under normal climatic conditions may not be suitable in current times with more climate variability. Our experiment investigated the effect of planting date (early, historic-optimum, and late), row spacing (19 and 25 cm), variety (Goodstreak, Robidoux, and Wesley), and seed rate (1.8, 2.1, 2.3, 2.4, 2.6, 2.8, 3.1, and 3.4 M seeds ha−1) on winter wheat grain yield and yield components. The seeding rate was nested within row spacing in nested-factorial design. A nested-factorial treatment design was used with testing at several locations in Nebraska across two years. Variety had a substantial effect on winter wheat grain yield (p < 0.05). Variety also had a substantial interaction effect with planting date and row spacing 50% of the time (p ≤ 0.01). At Hemingford, for example, Wesley planted at 19 cm had 5.9% more yield when compared to Robidoux planted at 19 cm (5.5 Mg ha−1). Similarly, biomass was influenced by variety across sites (p < 0.01), but a substantial interaction effect also occurred between planting date and variety at two of the three sites. Narrow row spacing (19 cm) led to significantly more tillers (6.9 M ha−1) when planted with Goodstreak at two of the sites. While planting date by itself did not affect any of the responses evaluated, this research highlights the importance of comprehensive and holistic approaches to wheat production in the High Plains. [ABSTRACT FROM AUTHOR]

Drought during the overwintering period threatens the emergence rate and restricts the yield under the "winter-seeded spring wheat" cultivated model in the Hetao Plain Irrigation District in Inner Mongolia. To address this issue, from 2017 to 2019, six treatments were set up in the field to study the emergence rate, growth attributes, grain quality, yield and its percentage of winter-seeded spring wheat. These treatments were the (1) application of water-retaining agents under winter sowing (WRA), (2) soil amendments under winter sowing (SA) and wheat seed presoaking with amino acid water-soluble fertilizer under winter sowing (SP), (3) straw mulching under winter sowing (SM), (4) film-mulching hole sowing under winter sowing (FMHS), (5) blank control under winter sowing (CKW) and (6) conventional blank control under spring sowing (CKS). The results showed that the emergence rate of winter-seeded treatment was lower than CKS treatment, the emergence of WRA, SA, SM, and FMHS treatment increased by 5.4%, 2.3%, 6.5% and 10.8% compared with CKW treatment, respectively. The winter-seeded treatment is earlier than CKS treatment in the growth process, in which FMHS treatment is between 12 d and 16 d earlier in the emergence period, between 13 d and 15 d earlier in the maturation period, between 8 d and 12 d earlier than the CKW treatment in the emergence period, and between 8 d and 10 d earlier in the maturity period. Compared with CKW treatment, WRA, SA, SM, FMHS and CKS treatments increased yield by 13.49%, 11.42%, 14.75%, 21.61% and 28.15%, respectively. FMHS treatment significantly reduced the total water consumption and significantly improved water use efficiency. The protein content, wet gluten content, sedimentation value, dough ductility and maximum resistance in CKS treatment were significantly lower than other winter sowing treatments. The protein percentage and wet gluten percentage in FMHS treatment were the highest, and the difference with CKW treatment was significant. In summary, film-mulching hole sowing in winter improves soil water and the emergence rate, significantly accelerates the growth process of wheat, increases yield and promotes grain quality. [ABSTRACT FROM AUTHOR]

Selenium (Se) biofortification by seed treatments has been poorly explored in wheat due to the difficulties in establishing seed treatments without compromising plant productive traits. We investigated the effects of Se seed soaking as a pre-sowing treatment in bread wheat. Five soaking periods and six Se concentrations were assessed on germination and seedling traits and compared to unsoaked seeds. Twelve hours of soaking was found beneficial for most tested Se concentrations. Then, we evaluated the effects of untreated, 0, 2.5 and 25 mM Se in 12 h seed soaking treatments along the wheat crop cycle under water-deficit (WD) and well-watered (WW) conditions in a pot experiment. Our results evidenced that 12 h of 2.5 mM Se soaking did not affect the germination percentage, and speed-up seedling emergence resulted in a considerable Se seed uptake. These plants also displayed enhanced antioxidant capacity and vegetative biomass accumulation, especially under WD. The treatment with 25 mM of Se negatively affected aerial biomass, suggesting potential toxicity. Physiological responses of Se-treated plants remained unchanged, as well as grain traits. Altogether, we propose that 12 h soaking with 2.5 mM Se is a promissory pre-sowing approach to enrich bread wheat grain and straw, particularly under water-limited environments. [ABSTRACT FROM AUTHOR]

Recently, large-scale agriculture has led to increasing crop production. To increase crop productivity in large-scale cropping systems, attempts have been made to make nano-fertilizers and deliver them to the crops by extension of nanotechnology. Hence, nano-fertilizers might be defined as nanoparticles that may directly assist in supplying essential nutrients for crop productivity. Seed germination is the first and the most susceptible stage in the plant's growing phases, so could be considered as an index to evaluate the effect of newly developed materials such as nanoparticles (NPs), providing useful information for researchers. In our experiments, germination tests have been carried out in Petri dishes containing wet filter paper and nano-primed seeds. We had biosynthesized seven nanoparticles in our previous studies including calcinated and non-calcinated zinc oxide, zinc, magnesium oxide, silver, copper, and iron nanoparticles. The effect of these biogenic nanoparticles and their counterpart metallic salts including zinc acetate, magnesium sulfate, silver nitrate, copper sulfate, and iron (III) chloride was studied on two popularly grown plants, wheat and flax, in laboratory conditions to obtain preliminary information for future field experiments. Germination percentage, shoot length, root length, seedlings length, root–shoot ratio, seedling vigor index (SVI), shoot length stress tolerance index (SLSI), and root length stress tolerance index (RLSI) were calculated on the second and seventh days of the experiment. According to the results, the response of the plants to metal containing nanoparticles and metal salts mainly depend on the type of the metal, plant species, concentration of the NP suspension or salt solution, condition of the exposure, and the stage of growth. [ABSTRACT FROM AUTHOR]

Exploiting new genetic resources is an effective way to achieve sustainable wheat production. To this end, we exposed wheat seeds of the "Punjab-11" cultivar to gamma rays. A total of 32 stable mutants (M7) were developed, followed by characterization by conducting multilocation trials over two seasons. Principal component analysis (PCA) showed that the first six components accounted for 90.28% of the total variation among the plant height, tillers per plant, 1000-kernel weight, grain yield, and quality traits. All mutants were grouped into three clusters based on high yield index values. The genotype by trait (GT) bi-plot revealed significant associations between yield and its components among the mutants. Positive correlations were estimated for tillers per plant, plant height, 1000-kernel weight, and grain yield; however, yield components showed negative associations with protein, moisture, and gluten contents. The mutant lines Pb-M-59 waxy, Pb-M-1272 waxy, Pb-M-2260, Pb-M-1027 waxy, Pb-M-1323 waxy, and Pb-M-1854 exhibited maximum grain yield, 1000-grain weight, and tillers per plant values. Likewise, Pb-M-2725, Pb-M-2550, and Pb-M-2728 were found to be the best mutant lines in terms of grain quality; thus, the use of gamma radiation is effective in improving the desirable traits, including yield and grain quality. It is suggested that these traits can be improved beyond the performance of corresponding traits in their parent genotypes. The newly produced mutants can also be used to explore the genetic mechanisms of complex traits in the future. [ABSTRACT FROM AUTHOR]

Water uptake is a seminal process in seed germination. Salt and polyethylene glycol (PEG) are known to retard seed germination rates and percentages, which is often attributed to osmotic effects. Here, we quantified water uptake in wheat seeds killed with a hot needle, finding evidence of three distinct water uptake pools. The fast pool was unaffected by salt, and likely represents cell walls and other apoplastic material. Water uptake into the medium and slow pools was slowed by salt addition, with the medium pool thought to be cellular, while the slow pool is presumably related to endosperm hydration. Salt caused a minor decrease in the water uptake rates and maximum seed water content, while PEG strongly suppressed both parameters. Seeds transferred between water and salt solutions followed the water uptake trajectories of the solution into which they were transferred. Seeds transferred from PEG to water achieved final seed water contents similar to water control seeds, while seeds transferred from water to PEG achieved significantly higher final water contents than PEG controls. This work confirms that salt and PEG have distinct effects on water uptake by wheat seeds. [ABSTRACT FROM AUTHOR]

The potential protective role of priming wheat seeds with maize green extract (MGE) against the stress effects of drought was studied. Pretreatment using MGE, MGE enriched with polyamines (MGEPA), and drought treatments (irrigation deficit of 30% (severe drought) or 60% (moderate drought) versus 90% relative water content of soil as a control) were applied in a factorial completely randomized design. Under moderate drought, pretreatment with MGEPA outperformed MGE and control, while severely stressed plants died even with pretreatments. Both extracts enhanced normal plant growth and yield and mitigated the deleterious effect of moderately stressed plants. Application of both extracts markedly increased photosynthetic efficiency, membrane stability, relative water content, and accumulation of antioxidants, osmoprotectants, trans- and cis-zeatin, polyamines, and their gene expressions, while levels of superoxide (O2•−) and hydrogen peroxide (H2O2), lipid peroxidation, and electrolyte leakage were decreased. Enzymatic antioxidants and glyoxalase system activities were improved in moderately stressed plants and were further improved with pretreatment with both extracts, thus protecting plants from oxidative damage by up-regulation of the ascorbate–glutathione cycle. Glycine betaine, soluble sugars, and proline levels were greatly increased in pretreated plants, thus maintaining membrane stability and photosynthetic efficiency. The interaction between drought and pretreatment using MGEPA was significant in growing wheat plants in dry environments with 60% relative water content of soil. [ABSTRACT FROM AUTHOR]

Although wheat and maize are the major economically important cereal crops and staple food sources in the world, their productivity is highly affected by excess salts in soil (salinity). Applications of multifarious halophilic plant growth-promoting rhizobacteria (PGPR) in saline soil protect the plants from osmotic damages and promote plant growth through the secretion of plant growth promoting (PGP) and osmolytes. In this study, Klebsiella variicola SURYA6—a PGPR—was evaluated for plant-growth-promotion and salinity amelioration in wheat and maize, and enrichment of soil nutrients. The results of the present study revealed that K. variicola SURYA6 grows luxuriously under high salinity stress conditions and produces copious amounts of three principal salinity ameliorating traits, such as 1 aminocyclopropane-1-carboxylate deaminase (ACCD), indole-3-acetic acid (IAA), exopolysaccharides (EPS), and osmolytes—such as proline, sugars, proteins, and amino acids. The isolate also exhibited sensitivity to a wide range of antibiotics, lack of hemolytic ability, and absence of catalase and oxidase activities confirming its nonpathogenic nature. Inoculation of wheat and maize seeds with this multifarious strain, improved the physicochemical properties of soil, improved seed germination by 33.9% and 36.0%, root length by 111.0%, 35.1%, shoot height by 64.8% and 78.9%, and chlorophyll content by 68.4% and 66.7% in wheat and maize seedlings, respectively, at 45 days after sowing (DAS) under salinity stress. The improvement in plant growth can be correlated with the secretion of PGP traits and improved, uptake of minerals such as nitrogen (N), phosphorus (P), sodium (Na), potassium (K), and magnesium (Mg). While amelioration of salinity can be the result of secretion of osmolytes and the change in pH from salinity to neutrality. This inoculation also significantly improved the soil nutrients under salinity stress conditions. Inoculation of K. variicola SURYA6, resulted in more improved growth and nutrients contents in plants and enriched soil nutrients under salinity stress as compared to normal (non-saline) conditions. Such multifarious strain can serve as a potent bio-inoculant for growth promotion of wheat and maize in saline soil. However, multi-year field trials under different agro-climatic conditions are required to confirm the bio-efficacy of K. variicola SURYA6. [ABSTRACT FROM AUTHOR]

Biological effects of extracts obtained from the seaweeds Hormophysa cuneiformis (J.F.Gmelin) P.C.Silva and Actinotrichia fragilis (Forsskål) Bùrgesen were investigated using wheat for the improvement of growth and amelioration of the negative effects of soil salinity. Exposure of plants to salt stress resulted in an overall decrease in growth, chlorophyll a and b, carotenoids and soluble sugars, as well as nutrient uptake (i.e., K, Ca and Mg) and K+/Na+ ratio. At the same time, increases were found in proline, total free amino acids, phenolic compounds, malondialdehyde (MDA), Na+ ions, as well as the activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Priming wheat seeds with H. cuneiformis and A. fragilis extracts mitigated the negative impacts of salinity by enhancing growth and all the above attributes except MDA and Na+. Treatments with H. cuneiformis or A. fragilis extracts resulted in an increased intensity of the polypeptide bands with 200, 159, 120, 40, and 22 KDa which were already apparent in the control. A. fragilis showed higher effectiveness than H. cuneiformis extracts under both control and stressed regimes. Our results highlight "biofertilizer" properties of two seaweeds and furnish mechanistic insight into their salinity-improvement action, which is pertinent for both applied and basic research. [ABSTRACT FROM AUTHOR]

Ultra-early seeding of spring wheat (Triticum aestivum L.) on the northern Great Plains can increase grain yield and grain yield stability compared to current spring wheat planting systems. Field trials were conducted in western Canada from 2015 to 2018 to evaluate the impact of optimal agronomic management on grain yield, quality, and stability in ultra-early wheat seeding systems. Four planting times initiated by soil temperature triggers were evaluated. The earliest planting was triggered when soils reached 0–2.5 °C at a 5 cm depth, with the subsequent three plantings completed at 2.5 °C intervals up to soil temperatures of 10 °C. Two spring wheat lines were seeded at each planting date at two seeding depths (2.5 and 5 cm), and two seeding rates (200 and 400 seeds m−2). The greatest grain yield and stability occurred from combinations of the earliest seeding dates, high seeding rate, and shallow seeding depth; wheat line did not influence grain yield. Grain protein content was greater at later seeding dates; however, the greater grain yield at earlier seeding dates resulted in more protein production per unit area. Despite extreme ambient air temperatures below 0 °C after planting, plant survival was not reduced at the earliest seeding dates. Planting wheat as soon as feasible after soil temperatures reach 0 °C, and prior to soils reaching 7.5–10 °C, at an optimal seeding rate and shallow seeding depth increased grain yield and stability compared to current seeding practices. Adopting ultra-early wheat seeding systems on the northern Great Plains will lead to additional grain yield benefits as climate change continues to increase annual average growing season temperatures. [ABSTRACT FROM AUTHOR]

Seed quality is accessed by appropriate seed germination, seed moisture contents, insect/pathogen infestations and seed vigor. Seed storage conditions are essential to protect from deterioration. In this study, knowledge and practices of wheat and cotton seeds storage were accessed among commercial seed growers (CSGs) and non-commercial seeds growers (NCSGs) in the Vehari District of Pakistan, while samples of stored wheat and cotton seeds were also collected to assess the quality of stored seeds. Stored seeds in the study area were contaminated by a variety of fungi, with infestation percentages reaching 13% for wheat and 20.7% for cotton in seeds from NCSGs, compared to 9% (wheat) and 9.5% (cotton) in seeds from CSGs. The majority of seed growers (75.0%) did not have any training on seed storage. The growers (60.9%) were unaware that seed should not be stored in closed polythene bags and most (62.2%) were not well aware about seed-borne crop diseases. Most growers did not maintain the temperature and humidity of storage rooms (82.7%) and did not calculate the seed rate before sowing after seed germination tests (87.2%). However, seed dressing with fungicides was implemented by most farmers (69.9%). Controlling the temperature of the seed storage was significantly influenced by growers' age, while controlling the humidity of the seed storage was significantly influenced by growers' farming experience. Seed dressing with fungicides was positively associated with the graduation level of growers, while checking seed maturity was positively associated with the higher education (Master's level) of growers. There was a lack of active information centers in the study areas and a lack of agricultural information provision to farmers. Awareness regarding bad-quality seeds should be initiated to increase growers' knowledge. Training programs for providing adequate knowledge to growers and skills in seed storage should be organized. Legislation regarding the seed business and seed storage protective measures should be enforced to minimize problems by a seed-borne inoculum and deterioration of seed quality. [ABSTRACT FROM AUTHOR]

In the coffee industry, several by-products are generated during the production and consumption of coffee and represent an important waste from an environmental viewpoint. For improving the knowledge about this issue, a laboratory vermicomposting study of coffee silverskin (CS) and spent coffee grounds (SCG) spiked with mature horse manure (HM) in different proportions and using earthworm Eisenia andrei was carried out. The 60-day study focused on biological parameters such as total biomass gain, growth rate, cocoon production, and mortality. This study also investigated whether the vermicompost obtained could be useful and lacked toxicity through a seed germination test using hybrid wheat seeds. Results showed a disparity depending on the type of residue and the mixture used. Best options were those treatments with a medium–low amount of residue; 25% for SCG and 25% or 50% for CS. In addition, lack of toxicity was confirmed in all treatments. In conclusion, it is possible to carry out a vermicomposting of SCG and CS with some specific features. [ABSTRACT FROM AUTHOR]

Wheat breeding continues to be an important component of agricultural innovations in the Great Plains region of the US. This paper reviews Oklahoma's wheat breeding program over the past several decades with an emphasis on the economic aspects and planning of future breeding strategies. Over the past century, Oklahoma's wheat yields have increased but the productivity gains have been offset in part due to increased yield variability. The shift to improved wheat varieties has resulted in increased susceptibility to plant disease and pest pressure. While a few varieties introduced over the past few decades have dominated the wheat seed market, recent trends indicate producers are adopting a more diverse range of wheat varieties. Producers' concerns have expanded beyond demands for high yielding varieties to include more market oriented needs to increase protein content and test weight. This paper identifies several key policy instruments that Oklahoma stakeholders and policy makers should consider when planning future breeding strategies. A critical need is to assist Oklahoma producers in adapting to climate change by developing drought and heat resistant wheat varieties. Marketing new varieties would be improved and revenue increased if more innovative pricing structures were adopted to better align producers' willingness to pay with seed development costs, which could then be re-invested in breeding. Research budgets, which have remained stagnant in real terms, will need to be increased to adequately address producers' needs into the 21st century. [ABSTRACT FROM AUTHOR]

Crop rotations dominated by winter annual crops and relying on the use of herbicides to control weeds have resulted in weed communities dominated by a few highly specialized species such as Alopecurus myosuroides. Integrated weed management (IWM) represents a sensible strategy to target such difficult weeds, through a combination of preventive, cultural, and direct means. In six field trials over three years, we tested the effect of stale seedbed preparation, winter wheat seed rate, and chemical weed control strategy on Alopecurus myosuroides control efficacy and variability in efficacy. The field experiments were carried out under reduced tillage practice and without pre-sowing use of glyphosate. Stale seedbed preparation alone reduced A. myosuroides infestation level by 25% on average. No clear effect was found of increasing winter wheat seed rate from 300 to 400 seeds m−2. A combination of stale seedbed preparation and herbicide treatment in autumn and spring was found to be synergistic, improving weed control efficacy significantly and moreover reducing the variability in control efficacy and hence the risk for weed control failure. [ABSTRACT FROM AUTHOR]

Hybrid breeding facilitates the exploitation of heterosis and it can result in significant genetic gains and increased crop yields. Inefficient cross-pollination is a major limiting factor that hampers hybrid wheat seed production. In this study, we examined the genetic basis of anther extrusion (AE), which is an important trait in increasing cross-pollination, and thus improving seed set on the female lines and hybrid wheat seed production. We studied 300 segregating F2 plants and F2:3 families that result from a cross of two elite spring wheat lines. We observed that F2 and F2:3 populations hold significant and continuous genetic variation for AE, which suggests its reliable phenotypic selection. Composite interval mapping detected three quantitative trait loci (QTL) on chromosomes 3A, 5A, and 5D. The QTL on chromosome 5A (i.e., QAe.cimmyt-5A) was of large-effect, being consistently identified across generations, and spanned over 25 cM. Our study shows that (1) AE possesses strong genetic control (heritability), and (2) the QTL QAe.cimmyt-5A that imparted on an average of 20% of phenotypic variation can be used for marker-assisted selection (MAS) in breeding programs. In addition, pyramiding the large-effect QTL for MAS could efficiently complement the phenotypic selection since it is relatively easy and cheap to visually phenotype AE. This study reports the first large-effect QTL for AE in spring wheat, endorsing the use of this analysis in current hybrid wheat breeding and future Mendelization for the detection of underlying gene(s). [ABSTRACT FROM AUTHOR]