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The brief longevity of tree peony blossoms constrains its ornamental value and economic worth. Gibberellins (GAs) are crucial in the modulation of flower senescence, and GA 20-oxidase (GA20ox), GA 3-oxidase (GA3ox), and GA 2-oxidase (GA2ox) catalyze the synthesis and deactivation of bioactive GAs. In Paeonia ostii, a total of three PoGA20ox, ten PoGA3ox, and twelve PoGA2ox proteins were identified and comprehensively analyzed. The analysis of the gene structures, conserved domains, and motifs revealed structural similarities and variances among the GA20ox, GA3ox, GA2ox-A, and GA2ox-B subfamilies. The synteny analysis indicated a scarcity of collinear blocks within the P. ostii genome, with no tandem or whole-genome duplication/segmental duplications found in PoGAoxs. The investigation into the binding of transcription factors to PoGAox promoters and the assessments of the expression levels suggest that PoGA2ox1 and PoGA2ox8.1 are promising candidate genes implicated in the regulation of floral senescence. Further, Pos.gene61099 (BPC6) and Pos.gene61094 (CIL2) appear to modulate PoGA2ox1 transcription in a positive and negative manner, respectively, while Pos.gene38359 (DDF1) and Pos.gene17639 (DREB1C) likely enhance PoGA2ox8.1's expression. This study lays a foundation for an in-depth understanding of PoGAox functions and the development of strategies to delay flower senescence in tree peony. [ABSTRACT FROM AUTHOR]

The brief longevity of tree peony blossoms constrains its ornamental value and economic worth. Gibberellins (GAs) are crucial in the modulation of flower senescence, and GA 20-oxidase (GA20ox), GA 3-oxidase (GA3ox), and GA 2-oxidase (GA2ox) catalyze the synthesis and deactivation of bioactive GAs. In Paeonia ostii, a total of three PoGA20ox, ten PoGA3ox, and twelve PoGA2ox proteins were identified and comprehensively analyzed. The analysis of the gene structures, conserved domains, and motifs revealed structural similarities and variances among the GA20ox, GA3ox, GA2ox-A, and GA2ox-B subfamilies. The synteny analysis indicated a scarcity of collinear blocks within the P. ostii genome, with no tandem or whole-genome duplication/segmental duplications found in PoGAoxs. The investigation into the binding of transcription factors to PoGAox promoters and the assessments of the expression levels suggest that PoGA2ox1 and PoGA2ox8.1 are promising candidate genes implicated in the regulation of floral senescence. Further, Pos.gene61099 (BPC6) and Pos.gene61094 (CIL2) appear to modulate PoGA2ox1 transcription in a positive and negative manner, respectively, while Pos.gene38359 (DDF1) and Pos.gene17639 (DREB1C) likely enhance PoGA2ox8.1’s expression. This study lays a foundation for an in-depth understanding of PoGAox functions and the development of strategies to delay flower senescence in tree peony.

Tree peony (Paeonia ostii) is an important ornamental plant with high oil and medical values and is widely distributed in China. In order to explore the effects of sucrose treatment on the growth of the P. ostii seeds and identify key genes involved in sucrose metabolism and transport during the early development of the P. ostii seeds, the comparative transcriptome sequencing of the P. ostii seed coats and endosperms at 50 days after flowering (DAF) with and without sucrose supply were conducted using Illumina HiSeq platform. A total of 97,976 unigenes were obtained and annotated with seven public function protein databases. There were 22,779, 22,632, 16,700, and 36,233 differentially expressed genes (DEGs) identified in the four comparisons, respectively. A total of 77 DEGs associated with the sucrose metabolism and transporter were identified in this study. Among them, sucrose transporter protein (SUT) and sweet sugar transporter (SWEET) were predicted to participate in sucrose transport from seed coat to embryo, and invertase (INV) was identified to be involved in sucrose degradation. In addition, sucrose synthase (SUS) and sucrose phosphate synthase (SPS) related to sucrose synthesis were highly expressed in seeds with sucrose supply. Finally, nine differentially expressed genes were selected randomly to analyze their expression levels using qRT-PCR, which were consistent with the transcriptome sequencing results. Above all, these results and analyses provide valuable gene resources and directions for further studying the effects of sucrose supply on the molecular mechanism of the sucrose metabolism and transporter during the development of P. ostii seeds. [ABSTRACT FROM AUTHOR]

Tree peony (Paeonia ostii) is an important ornamental plant with high oil and medical values and is widely distributed in China. In order to explore the effects of sucrose treatment on the growth of the P. ostii seeds and identify key genes involved in sucrose metabolism and transport during the early development of the P. ostii seeds, the comparative transcriptome sequencing of the P. ostii seed coats and endosperms at 50 days after flowering (DAF) with and without sucrose supply were conducted using Illumina HiSeq platform. A total of 97,976 unigenes were obtained and annotated with seven public function protein databases. There were 22,779, 22,632, 16,700, and 36,233 differentially expressed genes (DEGs) identified in the four comparisons, respectively. A total of 77 DEGs associated with the sucrose metabolism and transporter were identified in this study. Among them, sucrose transporter protein (SUT) and sweet sugar transporter (SWEET) were predicted to participate in sucrose transport from seed coat to embryo, and invertase (INV) was identified to be involved in sucrose degradation. In addition, sucrose synthase (SUS) and sucrose phosphate synthase (SPS) related to sucrose synthesis were highly expressed in seeds with sucrose supply. Finally, nine differentially expressed genes were selected randomly to analyze their expression levels using qRT-PCR, which were consistent with the transcriptome sequencing results. Above all, these results and analyses provide valuable gene resources and directions for further studying the effects of sucrose supply on the molecular mechanism of the sucrose metabolism and transporter during the development of P. ostii seeds.