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Radiation use efficiency (RUE) is difficult to estimate and unreasonable to perform on a small plot scale using traditional techniques. However, the increased availability of Unmanned Aerial Vehicles (UAVs) provides the ability to collect spatial and temporal data at high resolution and frequency, which has made a potential workaround. An experiment was completed in Iowa to (i) demonstrate RUE estimation of soybean [Glycine max (L.) Merr.] from reflectance data derived from consumer-grade UAV imagery and (ii) investigate the impact of foliar fungicides on RUE in Iowa. Some fungicides are promoted to have plant health benefits beyond disease protection, and changes in RUE may capture their effect. Frogeye leaf spot severity did not exceed 2%. RUE values ranged from 0.98 to 1.07 and 0.96 to 1.12 across the entire season and the period post-fungicide application, respectively, and fell within the range of previously published soybean RUE values. Plots treated with fluxapyroxad + pyraclostrobin had more canopy cover (p = 0.078) compared to the non-treated control 133 days after planting (DAP), but yields did not differ. A &ldquo, greening effect&rdquo, was detected at the end of the sample collection. RUE estimation using UAV imagery can be considered a viable option for the evaluation of management techniques on a small plot scale. Since it is directly related to yield, RUE could be an appropriate parameter to elucidate the impact of plant diseases and other stresses on yield.