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The GRAS protein family is involved in plant growth and development, plant disease resistance, and abiotic stress response. Although the GRAS protein family has been systematically studied and reported in many plants, it has not been reported in oat, an excellent foodstuff crop of Gramineae. We identified 90 AsGRAS genes and all of the AsGRAS genes were randomly distributed on 21 chromosomes with 6 tandem duplicated genes and 49 pairs of segmental duplications, which may be the main reason for the expansion of the GRAS gene family. According to the phylogenetic tree, 90 AsGRASs were classified into 10 distinct subfamilies. Gene structure revealed introns varying from zero to seven, and all genes have conserved motifs and GRAS structure domain. Protein–protein interaction and miRNA prediction analysis showed that AsGRAS proteins mainly interacted with GA signalling, cell division, etc., and that the AsGRAS genes were targeted by miRNA171. RNA-seq and qRT–PCR data showed that GRAS genes were expressed at different growth and developmental stages and under different abiotic stresses in oat, indicating the potential role of GRAS genes in promoting growth and stress tolerance in oat. Overall, our evolutionary and expression analysis of AsGRAS genes contributes to the elucidation of a theoretical basis for the GRAS gene family. Moreover, it helped reveal gene function and laid the foundation for future agricultural improvement of oats based on functional properties. [ABSTRACT FROM AUTHOR]