Drought and low soil fertility are the main problems in the development of sorghum crops as food, feed, and industrial raw materials (ethanol). The application of phosphorus fertilizer (P) is expected to increase stem biomass, juice volume, Brix content, and grains production. The purpose of this study was to assess the various effects of P fertilizer application on growth, yield, Brix sugar content, and biomass various varieties of sorghum. The study was carried out at Indonesia Cereals Research Institute Experimental Station, Maros South Sulawesi. The randomized block design (RBD) of three replications with two factors was used in the experiment. The first factor was the sorghum variety (Super 1, Super 2, and Numbu). The second factor were phosphorus fertilizer (P): P1 = without phosphorus fertilizer, P2 = 25 kg/ha P fertilizer, P3 = 50 kg/ha P fertilizer, P4 = 75 kg/ha P fertilizer, P5 = 100 kg/ha P fertilizer and P6 = 125 kg/ha P fertilizer. The reference dose of fertilizer was 120 kg/ha N, 36 kg/ha P (P2O5), and 90 kg/ha KCl (K2O). Data were analyzed using ANOVA analysis of variance, the significantly different data were analyzed further using the Duncan test with an error rate of 5%. The results were shown that the application of P fertilizer at a dose of 50 kg/ha produces the highest grain yield in Super 1 varieties (2.73 t / ha), Numbu varieties (1.93 t/ha) P fertilizer at a dose of 50 kg/ha and Super 2 variety produced (3.76 t/ha) P fertilizer at a dose of 125 kg/ha. The highest sugar content of Brix was obtained in Super 1 P fertilizer at a dose of 100 kg/ha (12.95%), Super 2 P fertilizer 125 kg/ha (15.61%), and Numbu by applying P fertilizer 125 kg/ha (14.19%)., Kondisi lahan yang kering dan kesuburan tanah yang rendah menjadi permasalahan utama dalam pengembangan sorgum sebagai bahan pangan, pakan dan bahan baku industri (ethanol). Pemberian pupuk fospor (P) diharapkan dapat meningkatkan biomas batang, volume nira, kadar brix dan biji sorgum. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh berbagai dosis pemberian pupuk P terhadap pertumbuhan, hasil, kadar gula brix dan biomas beberapa varietas sorgum. Penelitian di laksanakan di Kebun Percobaan Balai Penelitian Tanaman Serealia, Maros Sulawesi Selatan. Rancangan yang digunakan dalam percobaan ini adalah rancangan acak kelompok (RAK) tiga ulangan dengan 2 faktor. Faktor pertama adalah varietas (Super 1, Super 2 dan Numbu). Faktor kedua adalah penambahan pupuk tunggal Posfor (P) : P1= tanpa pupuk fosfor, P2= 25 kg/ha pupuk P, P3= 50 kg/ha pupuk P, P4= 75 kg/ha pupuk P, P5= 100 kg/ha pupuk P dan P6= 125 kg/ha pupuk P. Dosis pupuk acuan yang digunakan adalah 120 kg/ha N, 36 kg/ha P (P2O5) dan 90 kg/ha KCl (K2O). Data dianalisis menggunakan analisis sidik ragam Anova, apabila berpengaruh nyata dilakukan uji lanjut Duncan dengan taraf kesalahan 5%. Hasil penelitian menunjukkan pemberian pupuk P dosis 50 kg/ha menghasilkan panen biji tertinggi pada varietas Super 1 (2.73 t/ha), Numbu (1.93 t/ha) dan pupuk P dengan dosis 25 kg/ha menghasilkan bobot panen tertinggi pada varietas Super 2 (3.76 t/ha). Kadar gula brix tertinggi diperoleh pada varietas Super 1 dosis P 100 kg/ha (12.95%), Super 2 dosis P 125 kg/ha (15.61%) dan Numbu dosis P 125 kg/ha (14.19%).