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Strawberry is small fleshy fruit, with high values of biochemical contents. The essential effects of strawberry in human health have been approved due to significant amount of its vitamin C, antioxidant, phenolic compounds and anthocyanin. Wild strawberry (Fragaria vesca L.), from Rosaceae family, grows wildly in some north regions of Iran and have been traditionally used for food and medicinal purposes. In this study, five wild strawberry populations from Gilan province in the north of Iran, were used to investigate the value of chemical traits including; total soluble solid (TSS), titratable acidity (TA), vitamin C and bioactive content; antioxidant activity, total anthocyanin and total phenolic content. Among these five populations, Masoleh population showed the highest value of assessing factors while Ardeh population had the lowest. The total phenolic content was statistically correlated to the antioxidant activity (r = + 0.99; p < 0.05) and total anthocyanin content (r = +0.93; p < 0.05). Correlation matrix of biochemical contents showed that the climate condition (altitude, longitude, latitude) of collected fruits can be related to the biochemical quantity. According to the results, high concentration of the antioxidant activity was presented in the Iranian wild strawberries. Current study suggests that environmental factors can change the antioxidant capacity and biochemical compositions of wild strawberry. To sum it up, wild strawberry populations of Iran can be considered as a good source for breeding program, plus it may be introduced as a fruit with great value for human health. [ABSTRACT FROM AUTHOR]