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The fatty acid desaturase 2 (fad2) gene was proven to be a major locus for high oleic acid (C18:1). Brassica napus is an amphidiploid species originating from a spontaneous hybridization of Brassica rapa and Brassica oleracea. B. napus contains multiple copies in genome for most of the genes, including fad2 genes. The research cloned nine fad2 genes from 3 varieties of B. rapa and 3 varieties of B. oleracea, respectively. Alignment of the nine fad2 sequences from B. rapa and B. oleracea detected 6 single nucleotide polymorphic sites, which resulted in 6 amino-acid substitutions. The nucleotide substitutions at position 743 bp in the fad2-A gene and position 947 bp in the fad2-C gene were used as 3' end of al ele-specific primers. In use of the allele-specific primers to amplify fad2 gene, we could identify if the fad2 gene originated from A genome or C genome. Besides, the research found that fad2 genes in C genome are more conserved in evolutionary process than those in A genome. The fad2 expression data reported in this study revealed that fad2-A from B. rapa was not only expressed in siliques same as fad2C from B. oleracea, but also expressed in a high level in stems. Not even the less, fad2 gene from B. napus was expressed higher in roots and flowers. Al these results provided evidences that fad2, though it was expressed differently in B. rapa and B. oleracea, but it was regulated by the same approach in B. napus. [ABSTRACT FROM AUTHOR]

The establishment of crop yield estimating model based on microwave and optical satellite images can conduct the mutual verification of the accuracy of the reported crop yield and the precision of the estimating model. With Shou County and Huaiyuan County of Anhui Province as the experimental fields of winter wheat producing areas, the linear winter wheat yield estimating models were established by adopting backscattering coefficient and Normalized Difference Vegetation Index (NDVf) based on images from the synthetic aperture radar (SAR)—RDARSAT-2 and HJ satellite photographed in mid-April and early May, 2014, and then comparisons were conducted on the accuracy of the yield estimating models. The accuracies of the yield estimating models established using co-polarized (HH) arid cross-polarized (HV) modes of SAR in Jiangou Town, Shou County were 68.37% and 74.01%, respectively, while the accuracies in Longkang Town, Huaiyuan County were 63.10% and 69.10%, respectively. Accuracies of yield estimating models established by HJ satellite data were 69.52% and 66.43% in Shou County and Huaiyuan County, respectively. Accuracies of winter yield estimating model based on HJ satellite data and that based on SAR were closed, and the yield difference of winter wheat in the lodging region was analyzed in detail. The model results laid the foundation and accumulated experience for the verification, parameters correction and promotion of the winter wheat yield estimating model. [ABSTRACT FROM AUTHOR]

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Copyright of Agricultural Science & Technology is the property of Hunan Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)