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[Objective] This study aimed to investigate the influence of high temperature on the expression of heat shock transcription factor AtHsfAla in different genotypes of Arabidopsis. [Method] Arabidopsis plants overexpressing heat shock transcription factor AtHsfAla were used as experimental materials and treated under high temperature at 39 °C for 1 min and 5 min; total RNA of AtHsfAla was extracted, and the reverse transcription and amplification were conducted using RT-PCR technology, the amplification products were detected by electrophoresis. [Result] The expression levels of AtHsfAla in Arabidopsis plants overexpressing heat shock transcription factor AtHsfAl a at high temperature and room temperature were higher than wild-type Arabidopsis; the expression levels of AtHsfAla in both wild-type Arabidopsis and transgenic Arabidopsis plants overexpressing heat shock transcription factor AtHsfAl a at high temperature of 39 °C were higher than that at room temperature of 25 °C, but the expression levels of AtHsfAla in wild-type Arabidopsis and transgenic Arabidopsis plants overexpressing heat shock transcription factor AtHsfAla varied little after high temperature treatment at 39 °C for 1 min or 5 min. [Conclusion] The expression of AtHsfAla is induced rapidly by high temperature, thus regulating the expression of early adversity-resistant genes. This study will lay the foundation demonstrating the mechanism of Arabidopsis heat shock transcription factor AtHsfA1a. [ABSTRACT FROM AUTHOR]