1. Multiwalled carbon nanotubes co-delivering sorafenib and epidermal growth factor receptor siRNA enhanced tumor-suppressing effect on liver cancer
- Author
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Yuliang Feng, Shanwen Chen, Li Guo, Lijun Wan, Xiaohua Yan, Lingyan Lian, Youwen Hu, Hongyan Huang, Degejirifu, Moran Zhang, Qian Yang, Zhili Wen, and Kedong Xu
- Subjects
Sorafenib ,Aging ,Clone (cell biology) ,Mice, Nude ,Antineoplastic Agents ,Apoptosis ,multiwalled carbon nanotubes ,liver cancer ,Mice ,Drug Delivery Systems ,In vivo ,medicine ,Animals ,Humans ,Epidermal growth factor receptor ,RNA, Small Interfering ,Cell Proliferation ,biology ,Nanotubes, Carbon ,Cell growth ,Chemistry ,Liver Neoplasms ,Hep G2 Cells ,Cell Biology ,medicine.disease ,Molecular biology ,In vitro ,ErbB Receptors ,Liver ,biology.protein ,epidermal growth factor receptor ,Liver cancer ,Neoplasm Transplantation ,Research Paper ,medicine.drug - Abstract
Objective This study aimed to investigate the effects of multiwalled carbon nanotubes (MWNTs) co-delivering sorafenib (Sor) and epidermal growth factor receptor (EGFR) siRNA (MWNT/Sor/siRNA) on tumor growth in liver cancer (LC). Results MWNT/Sor/siRNA was proved to possess increased Sor release, high siRNA stability, and enhanced cellular uptake. In addition, MWNT treatment has few effects on cell proliferation and apoptosis in HepG2 cells; however, MWNT/Sor/siRNA treatment significantly inhibited clone number and induced cell apoptosis, which shows a more favorable antitumor effect than MWNT/Sor and free Sor and free siRNA in HepG2 cells. Moreover MWNT/Sor/siRNA treatment has the most significant antitumor effect in vivo. Conclusions MWNT/Sor/siRNA exhibited a superior antitumor effect in vitro and in vivo. Methods The MWNT/Sor and MWNT/Sor/siRNA were prepared, and then the morphologies of MWNT/Sor/siRNA were analyzed. In vitro Sor release assay, siRNA stability and cellular uptake of MWNT/Sor/siRNA were performed as well. Next, the effects of MWNT, free Sor, free siRNA, MWNT/Sor and MWNT/Sor/siRNA were evaluated by colony-forming assay, and cell apoptosis assay in HepG2 cells. Meanwhile, the level of EGFR and proteins associated with apoptosis was tested. Furthermore, the anti-tumor effects of MWNT/Sor/siRNA on LC xenograft mice were also unraveled.
- Published
- 2021
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