1. Aging and calorie restriction regulate the expression of miR-125a-5p and its target genes Stat3, Casp2 and Stard13
- Author
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Roman V. Kondratov, Kuldeep Makwana, Jey Sabith Ebron, Nikkhil Velingkaar, Sonal A. Patel, and Girish C. Shukla
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STAT3 Transcription Factor ,0301 basic medicine ,Aging ,Calorie ,Calorie restriction ,Mice ,03 medical and health sciences ,0302 clinical medicine ,longevity ,Downregulation and upregulation ,Transcription (biology) ,microRNA ,Animals ,STAT3 ,Gene ,Caloric Restriction ,biology ,Tumor Suppressor Proteins ,Caspase 2 ,MicroRNA ,calorie restriction ,Cell Biology ,Cell biology ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Liver ,030220 oncology & carcinogenesis ,biology.protein ,transcription ,Research Paper ,Mir 125a - Abstract
Calorie restriction (CR) is a dietary intervention known to delay aging. In order, to understand molecular mechanisms of CR, we analyzed the expression of 983 MicroRNAs (miRNAs) in the liver of female mice after 2 years of 30% CR using micro-array. 16 miRNAs demonstrated significant changes in their expression upon CR in comparison with age-matched control. mmu-miR-125a-5p (miR-125a-5p) was significantly upregulated upon CR, and in agreement with this, the expression of mRNAs for its three predicted target genes: Stat3, Casp2, and Stard13 was significantly downregulated in the liver of CR animals. The expression of precursor miRNA for miR-125a-5p was also upregulated upon CR, which suggests its regulation at the level of transcription. Upon aging miR-125a-5p expression was downregulated while the expression of its target genes was upregulated. Thus, CR prevented age-associated changes in the expression of miR-125a-5p and its targets. We propose that miR-125a-5p dependent downregulation of Stat3, Casp2, and Stard13 contributes to the calorie restriction-mediated delay of aging.
- Published
- 2017
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