Search

Your search keyword '"J. K. Nielsen"' showing total 2 results
Start Over Author "J. K. Nielsen" Journal agents and actions
2 results on '"J. K. Nielsen"'

1. Unusual effects of the ionophore A23187 at suboptimal concentrations in mast cell histamine release

Author

J. K. Nielsen and Nina Grosman

Subjects
Male, Immunology, Ionophore, chemistry.chemical_element, In Vitro Techniques, Calcium, Toxicology, Histamine Release, chemistry.chemical_compound, medicine, Animals, Potency, Dimethyl Sulfoxide, Pharmacology (medical), Mast Cells, Incubation, Calcimycin, Standard buffer, Pharmacology, Rats, Inbred Strains, Mast cell, Rats, Solvent, medicine.anatomical_structure, chemistry, Biochemistry, Biophysics, Histamine
Abstract

Certain peculiarities are observed in connection with histamine release induced by suboptimal concentrations of the ionophore A23187. The potency of the ionophore can increase by a factor of 3 when added to the incubation medium from solutions in either DMSO or buffer without added calcium as compared with addition from standard buffer. The effect was not due to the solvent per se but may relate to physicochemical conditions facilitating the ionophore's access to cellular domains.

Published
1990
Full Text
View/download PDF

2. Influence of TMB-8 and calmidazolium on phorbol ester promoted histamine release from isolated rat mast cells

Author

J. K. Nielsen and Nina Grosman

Subjects
Male, medicine.medical_specialty, Allergy, Immunology, Ionophore, Oxidative phosphorylation, Pharmacology, In Vitro Techniques, Toxicology, Histamine Release, Phorbol ester, chemistry.chemical_compound, Antigen, Calmodulin, Internal medicine, Gallic Acid, Phorbol Esters, medicine, Animals, Pharmacology (medical), Mast Cells, Protein kinase C, Calcimycin, Chemistry, Imidazoles, Rats, Inbred Strains, Mast cell, medicine.disease, Calcium Channel Blockers, Rats, Endocrinology, medicine.anatomical_structure, Histamine
Abstract

The effects of TMB-8 and calmidazolium were investigated on mast cell responses believed to be mediated by protein kinase C, i.e. histamine release induced by TPA (tetradecanoyl-phorbol-acetate) in combination with sub-threshold concentrations of the ionophore A23187 and with antigen. Inhibition with both drugs was found in the same concentration range as observed earlier and could be counteracted by glucose, indicating an impaired oxidative energy production. Hence, the test drugs do not reveal protein kinase C selectivity.

Published
1990

Catalog

Books, media, physical & digital resources
See catalog results