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To evaluate the combined cytotoxicity effects between functionalized multi-walled carbon nanotubes (MWCNTs-PC) and cigarette smoke solution (CSS), 16-HBE cells was used as the target cells and exposed to various concentrations of MWCNTs-PC and CSS combined together. Cell proliferation, cell apoptosis, DNA damage were detected by Methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, single cell gel electrophoresis assay (SCGE) and micronuclear assay, respectively. The dose-dependent cytotoxic and genetic effects of CSS were found in our study. However, compared to the control group, the MWCNTs-PC exposed groups showed no significant difference in all concentration, with or without CSS exposure. It suggests that the MWCNTs-PC did not influence cellular toxicity or DNA damage of CSS on 16-HBE cells. No combined cytotoxic effects between NWCNTs-PC and CSS were found in this study.

The widespread explored application of water soluble carbon nanotubes makes it important to understand their potential toxic effects on health. This study investigates the effects of phosphoryl choline grafted water soluble multi-walled carbon nanotubes (MWCNTs-PC) on human bronchial epithelial (16-HBE) cells by different cytotoxicity methods in vitro. Various concentrations of MWCNTs-PC were incubated with 16-HBE cells, the effects of cell proliferation, cell apoptosis, cell cycle and DNA damage were detected by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, single cell gel electrophoresis assay (SCGE) and micronuclear assay, respectively. Compared with the control group, there were no significant differences in the changes of cell proliferation, cell apoptosis, cell cycle and DNA damage. Within the experimental concentrations of MWCNTs-PC, no obviously cytotoxicity and DNA damage was observed on 16-HBE cells in this study.