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Effects of enhanced ultraviolet B (UV-B, 280-320 nm) on macroalgae have gained particular attention in recent years. We investigated the changes in the growth, physiological and biochemical characteristics of C. ocellatus under UV-B radiation in this study. The results showed that UV-B could inhibit the growth of C. ocellatus (pC. ocellatus were not obvious. The soluble proteins content and MDA content in C. ocellatus were increased by UV-B radiation, and the increase of MDA content was extraordinary significant. The SOD activity and CAT activity were increased when the intensity of UV-B radiation was 7.2 J/m2, while the SOD activity and CAT activity were decreased when the intensity of UV-B radiation was 21.6 J/m2.