1. Is there room for luminal-basal urothelial cell population quantification?
- Author
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Passos GR, Camargo JA, Ferrari KL, Franch GC Jr, Nowill AE, and Reis LO
- Subjects
- Animals, Cell Count, Cell Separation methods, Collagenases, Epithelial Cells ultrastructure, Flow Cytometry, Male, Peptide Hydrolases, Random Allocation, Rats, Specimen Handling methods, Epithelial Cells classification, Urothelium cytology
- Abstract
Purpose: Three cell layers compose the urothelium: basal, intermediate and luminal ("umbrella cells") and different diseases might arise from different cell populations. The aim of this study is to analyze the quantification ability of such cell populations by using four different protocols., Methods: Twenty male rats (Wistar) were randomized in four groups of five animals: scraping, enzymatic 30, 45 and 60minutes. The cells were isolated, analyzed by flow cytometer and data processed by BD FACSDIVA™ software., Results: The urothelium was separated in two cell populations that are different in size and complexity. The group that showed more efficiency in cells dissociation and cells separation was enzymatic protocol 45minutes., Conclusions: Enzymatic protocol 45minutes was able to isolate urothelial cell populations and might be explored as potential prognostic tool, patient selection and therapeutic target in urothelial diseases. Future studies should validate the potential clinical application to the proposed rational of luminal-basal paradigm in the urothelial cancer as hope for individualized approach., (Copyright © 2017 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2017
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