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Batai virus was isolated from the blood sample of one sentinel piglet out of 34 used to monitor the activity of the Japanese encephalitis (JE) virus at Madikere village in Kolar district, Karnataka State, India. This is the first report of the isolation of Batai virus from a mammal.

A pathogenic agent designated AV 172 was isolated from the blood of a Reed Warbler (Acrocephalus scirpaceus) out of 767 samples from birds belonging to 35 species and 14 families. The birds (largely wetland passerines) were captured in the reed-belt littoral of Nesyt fishpond in southern Moravia, Czechoslovakia, during the years 1984 to 1987. Virus AV 172 has been found to represent probably a new species (designated virus "Sedlec") of family Bunyaviridae. Sedlec virus is pathogenic to suckling and adult mice when inoculated intracerebrally (i.c.) but not intraperitoneally (i.p.) and its ether-sensitive spherical particles measures 90-100 nm.

Aedes aegypti mosquitoes originating from different colonies were infected with Tahyna virus by sucking on viraemic newborn mice (peak titre 10(2) X 5 mouse i.c. LD50/0.01 ml). The mosquitoes of laboratory strain "London" proved most susceptible (infection rate 74.2%, transmission rate 60%), while mosquitoes of the laboratory strain "Basel" and strain "Bangkok" (Southeast Asia) were less susceptible (infection rate 23.7 and 17.6%, respectively, transmission rate 6.0 and 10.0%, respectively). Mosquitoes of "Ifakara" strain (East Africa) were found resistant to Tahyna virus infection.