1. Detection of enteroviruses and hepatitis a virus RNA in cow milk by RT-PCR
- Author
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Özgür Çadirci, Zafer Yazici, Göknur Terzi, Harun Albayrak, Belgin Siriken, Semra Okur-Gumusova, and Ondokuz Mayıs Üniversitesi
- Subjects
General Veterinary ,viruses ,cow milk ,food and beverages ,virus diseases ,RNA ,biochemical phenomena, metabolism, and nutrition ,Biology ,Raw milk ,medicine.disease_cause ,Virology ,enteroviruses ,Microbiology ,Reverse transcription polymerase chain reaction ,fluids and secretions ,Real-time polymerase chain reaction ,Capsid ,hepatitis A virus ,medicine ,Enterovirus ,Dairy cattle ,Food contaminant - Abstract
WOS: 000279162300009 The aim of the study is to evaluate the precence of Hepatitis A virus (HAV) and enteroviruses (EV) in milk samples. Raw milk and whey samples (n=50), collected from eight different open air markets in Samsun (Turkey) were analysed for HAV and entero viruses by RT-PCR. Viral RNA was extracted using the rapid acid guanidinium thiocyanate-phenol-chloroform method. HAV primers were selected from sequences of the VP2 and VP4 capsid region. Entero virus primers were selected from the 5 non-translated region, which is the most conserved region in an enterovirus genome. Four samples (8%) raw milk were positive for EV RNA. However, HAV RNA was not determined the milk samples. This is the first report on the detection of HAV and EV RNA in cow milk samples in Turkey. This study highlights the interest for contamination by HAV and EV in milk samples from different areas.
- Published
- 2010
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