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Your search keyword '"Mandana Afsharpad"' showing total 2 results
Start Over Author "Mandana Afsharpad" Journal acta tropica
2 results on '"Mandana Afsharpad"'

1. Genetic structure of Plasmodium vivax isolates from two malaria endemic areas in Afghanistan

Author

Hoda Atta, Waqar Butt, Akram Abouie Mehrizi, Sedigheh Zakeri, Ghasem Zamani, Navid Dinparast Djadid, Mandana Afsharpad, Najibullah Safi, and Faezeh Ghasemi

Subjects
Male, Veterinary (miscellaneous), Plasmodium vivax, Protozoan Proteins, Antigens, Protozoan, Biology, Disease Outbreaks, parasitic diseases, Genotype, Genetic variation, Malaria, Vivax, Humans, Genotyping, Heat-Shock Proteins, Merozoite Surface Protein 1, Genetics, Genetic diversity, Molecular epidemiology, Afghanistan, Genetic Variation, biology.organism_classification, Virology, Infectious Diseases, Genetic marker, Insect Science, Female, Parasitology, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length
Abstract

In this study, the nature and extent of genetic diversity of Plasmodium vivax populations circulating in Afghanistan have been investigated by analyzing three genetic markers: csp, msp-1, and msp-3 alpha. Blood samples (n=202) were collected from patients presenting with vivax malaria from south-western (Herat) and south-eastern (Nangarhar) parts of Afghanistan, and analysed using nested-PCR/RFLP and sequencing methods. Genotyping pvmsp-1 revealed type 1, type 2 and recombinant type 3 allelic variants, with type 1 predominant in parasites in both study areas. The sequence analysis of 57 P. vivax isolates identified a total of 26 distinct alleles. Genotyping pvcsp gene showed that VK210 type (86.6%) is predominant in Afghanistan. Moreover, three major types of the pvmsp-3 alpha locus: type A, type B and type C were distinguished among Afghani isolates. The predominant fragments among Nangarhar and Herat parasites were type A (70.8% and 67.9%, respectively). PCR/RFLP products with Hha I and Alu I were detected 52 and 38 distinct variants among Nangarhar and Herat isolates, respectively. These results strongly indicate that the P. vivax populations in Afghanistan are highly diverse.

Published
2010
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2. Molecular monitoring of Plasmodium falciparum resistance to antimalarial drugs after adoption of sulfadoxine-pyrimethamine plus artesunate as the first line treatment in Iran

Author

Navid Dinparast Djadid, Mandana Afsharpad, Sakineh Pirahmadi, Sedigheh Zakeri, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP), This work was supported by a grant (no. 418) from Pasteur Institute of Iran to S. Zakeri., Mandana Afsharpad, Sedigheh Zakeri, Sakineh Pirahmadi, and Navid Dinparast Djadid

Subjects
Dihydropteroate, Drug Resistance, Protozoan Proteins, Artesunate, Drug resistance, Pharmacology, Iran, MESH: Genotype, chemistry.chemical_compound, 0302 clinical medicine, Gene Frequency, Chloroquine, MESH: Child, MESH: DNA Fingerprinting, 030212 general & internal medicine, Artemisinin, Malaria, Falciparum, Child, MESH: Protozoan Proteins, MESH: Plasmodium falciparum, MESH: Aged, MESH: Middle Aged, biology, MESH: Malaria, Falciparum, Middle Aged, Artemisinins, 3. Good health, Drug Combinations, Infectious Diseases, Pyrimethamine, MESH: Young Adult, MESH: Drug Resistance, [SDV.IMM]Life Sciences [q-bio]/Immunology, Polymorphism, Restriction Fragment Length, medicine.drug, Adult, Adolescent, Genotype, MESH: Pyrimethamine, Veterinary (miscellaneous), 030231 tropical medicine, Plasmodium falciparum, Mutation, Missense, 03 medical and health sciences, Antimalarials, Young Adult, parasitic diseases, MESH: Artemisinins, Sulfadoxine, medicine, MESH: Gene Frequency, Humans, Aged, Plasmodium falciparum Drug resistance pfdhfr pfdhps Sulfadoxine-pyrimethamine, MESH: Adolescent, MESH: Drug Combinations, MESH: Mutation, Missense, MESH: Humans, business.industry, MESH: Polymorphism, Restriction Fragment Length, MESH: Adult, medicine.disease, biology.organism_classification, Virology, MESH: Antimalarials, DNA Fingerprinting, Sulfadoxine/pyrimethamine, chemistry, Insect Science, MESH: Iran, Parasitology, business, Malaria, MESH: Sulfadoxine
Abstract

International audience; The main objective of this investigation was whether the combination therapy of sulfadoxine pyrimethamine (SP) plus artesunate (AS) protects against the spread of resistance to SP in malaria-endemic south-eastern Iran. Infected blood samples of Plasmodium falciparum (n=170) were collected during 2008-2010 after the adoption of SP-AS as the first line treatment in Iran. Four different genes of P. falciparum [dihydropteroate synthetase (pfdhps), dihydrofolate reductase (pfdhfr), chloroquine (CQ) resistance transporter (pfcrt K76T) and multidrug resistance1 (pfmdr1 N86Y)], associated with SP and CQ resistance were analyzed using PCR-RFLP methods. The result showed 4.1, 95.9 and 100% prevalence of pfdhfr 51I, 59R and 108N, respectively and the majority of patients (95.9%) were found to carry both 59R and 108N. The prevalence of single mutation at pfdhps 437G gene was 26.9% before the adoption of SP-AS, but as SP was used as the first line treatment; this mutation started to increase and reached a high level of 55.5% in 2008 (χ(2) test, P

Published
2011
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