1. Epidemiological investigation of Cryptosporidium in children with diarrhea in middle Inner Mongolia, China.
- Author
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Guo W, Xue X, Li R, Liang R, Wang Z, Qin J, Duan C, Chen T, and Yan X
- Subjects
- Humans, China epidemiology, Infant, Female, Male, Child, Preschool, Prevalence, Infant, Newborn, Child, DNA, Protozoan genetics, Seasons, Sequence Analysis, DNA, Genotype, Phylogeny, Cryptosporidium parvum genetics, Cryptosporidium parvum isolation & purification, Cryptosporidium parvum classification, DNA, Ribosomal genetics, DNA, Ribosomal chemistry, Cryptosporidiosis epidemiology, Cryptosporidiosis parasitology, RNA, Ribosomal, 18S genetics, Diarrhea epidemiology, Diarrhea parasitology, Feces parasitology, Cryptosporidium genetics, Cryptosporidium isolation & purification, Cryptosporidium classification
- Abstract
Cryptosporidia (Cryptosporidium) is a protozoan that is widely parasitic in the intestinal cells of humans and animals, and it is also an important zoonotic parasite. However, there is no epidemiological investigation on Cryptosporidium spp. infection in infants with diarrhea of Inner Mongolia, the largest livestock region in China. To investigate the prevalence of Cryptosporidium, 2435 fresh fecal samples were collected from children with diarrhea in Inner Mongolia Maternal and Child Health Care Hospital. Molecular characterization of Cryptosporidium was carried out based on its 18S rRNA and gp60 gene sequences. The overall prevalence was 12.85% (313/2435), and in Hohhot (12.15%), it was lower than that in the surrounding city (14.87%) (P < 0.05). Moreover, Cryptosporidium was detected in different seasons and sexes. Concerning the age of children with diarrhea, the prevalence of those age groups between 0 and 1 was obviously lower than others, and there were significant differences in the prevalence at different ages (P < 0.001). Analysis of the 18S rRNA gene sequence revealed that all the positive samples were Cryptosporidium parvum, and there were 5 subtypes (IIdA23G3, IIdA24G3, IIdA24G4, IIdA25G3, and IIdA25G4). To the best of our knowledge, the above subtypes have not been reported. Our results provide a relevant basis for control and education on food safety and foodborne illness prevention., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
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