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Transection of the common fibular (FIB) nerve caused an immediate reduction in the total amount of soleus (SOL) motor unit activity, which declined further during the following 10 days and then remained stable at less than half of normal values. In addition, there was an immediate reduction in median impulse rate from about 20.0 to 14.9 Hz followed by a return to normal values during the first 10 days. Short interval (3-4 ms) double discharges, occurring either in isolation or at the same regular intervals as single impulses, were observed 2-5 days after FIB nerve section in a few motor units. Brief, high frequency impulse bursts with interspike intervals of 12-16 ms were observed in a few units from the third day and until the end of the experiment (up to 31 days). It was not established whether the high frequency discharge pattern occurred only in a fixed population of the SOL motor units, or whether the units could switch between high and low frequency activity. Two months after FIB nerve section, the SOL muscle in the same leg contracted faster than normal (mean isometric twitch contraction time 29.6 ms, n = 4; vs. 38.7 ms, n = 8), contained a larger than normal percentage of type II fibres (13-36 vs. 0-0.2%) and weighed less than the contralateral SOL muscles (180 vs. 206 mg). SOL muscles (n = 4) in the opposite leg were comparable to normal SOL muscles except for a small reduction in mean isometric twitch contraction time (35.5 ms).

Soleus (SOL) and extensor digitorum longus (EDL) muscles in adult rats were kept denervated for 2 months by four repeated freezes at 2-week intervals of the sciatic nerve. Reinnervation was studied in the absence or presence of chronic muscle stimulation, starting 1 month before reinnervation began. In addition, reinnervation was studied in SOL muscles where a previously transplanted fibular (FIB) nerve had formed ectopic neuromuscular junctions outside the original endplate area. After repeated freezes only, reinnervation was complete judged by tension measurements and histochemical examinations in SOL (n = 7) and EDL (n = 8) muscles. In directly stimulated muscles reinnervation was incomplete, and the force tensions evoked from indirect stimulation was on average 87 (n = 5) and 82% (n = 5) of direct muscle stimulation in SOL and EDL muscles, respectively. Of ectopically innervated SOL muscle fibres, only 26% became reinnervated in 12 muscles. Denervation and reinnervation increased the number of muscle fibres in stimulated (n = 4) and unstimulated (n = 5) EDL muscles by 18 and 15%, respectively. In stimulated (n = 4) and unstimulated (n = 7) SOL muscles, on the other hand, the number of muscle fibres remained normal. The stronger suppression of reinnervation in ectopically reinnervated compared to chronically stimulated SOL fibres indicates that reinnervation can also be suppressed by activity independent influences from the foreign nerve.