1. Influence of Therapeutic Proton Beam on Glioblastoma Multiforme Proliferation Index — A Preliminary Study
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Teofila Książek, Pawel Olko, Tomasz Kajdrowicz, Natalia Mojżeszek, K. Rawojć, Jan Swakoń, Katarzyna Szewczyk, Wiktor Komenda, Anna M. Bogdali-Suślik, Agnieszka Panek, Monika Woźniak, Miroslaw Bik-Multanowski, Justyna Miszczyk, and Renata Kopeć
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Physics ,Proton ,Proliferation index ,protons ,General Physics and Astronomy ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,glioblastoma multiforme ,Nuclear magnetic resonance ,0103 physical sciences ,X-rays ,medicine ,010306 general physics ,0210 nano-technology ,Beam (structure) ,Glioblastoma - Abstract
The goal of the presented study was to compare the proliferation of different U118 MG and U251 MG glioblastoma cell lines irradiated with proton beam or X-rays in dose range 0.5-10.0 Gy. Cytokinesis-block micronucleus (CBMN) assay was carried out to study changes in proliferation presented as nuclear division index (NDI). Preliminary results suggest that protons and X-rays influence GBM (glioblastoma multiforme) cellular proliferation differently. Therapeutically, a decrease in NDI values with the increase in both types of radiation dose was found only for U251 MG cell line, thus can be classified as more radiosensitive than U118 MG cell line. Also for U251 MG GBM cell line, a therapeutic proton beam was more effective in inhibition of proliferation than X-rays. Genetic differences between GBM are supposed to be involved in the increased radiosensitivity, which is planned to be studied further by genes expression analysis.
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