1. Cryptosporidium parvum-Infected Neonatal Mice Show Gut Microbiota Remodelling Using High-Throughput Sequencing Analysis: Preliminary Results
- Author
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Karim Tarik Adjou, Myriam Thomas, Isabelle Vallée, Mohamed Mammeri, Bruno Polack, Eric Auclair, Christine Julien, Aurélie Chevillot, Thomas Pollet, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Phileo Lesaffre Animal Care, Institut National de la Recherche Agronomique (INRA), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
- Subjects
medicine.medical_specialty ,Firmicutes ,[SDV]Life Sciences [q-bio] ,030231 tropical medicine ,Cryptosporidiosis ,Gut microbiota ,Gut flora ,digestive system ,030308 mycology & parasitology ,Microbiology ,Actinobacteria ,Feces ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Medical microbiology ,RNA, Ribosomal, 16S ,CD-1 neonatal mouse model ,16S rDNA ,medicine ,Animals ,Intestinal Diseases, Parasitic ,Gene ,Cryptosporidium parvum ,0303 health sciences ,Bacteria ,biology ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,biology.organism_classification ,Gastrointestinal Microbiome ,3. Good health ,Hypervariable region ,Animals, Newborn ,Parasitology ,High-throughput sequencing occurrence - Abstract
International audience; Background During the last decade, the scientific community has begun to investigate the composition and role of gut microbiota in normal health and disease. These studies have provided crucial information on the relationship between gut microflora composition and intestinal parasitic infection, and have demonstrated that many enteric pathogen infections are associated with altered gut microflora composition. In this study, we investigated the effects of Cryptosporidium parvum infection (zoonotic protozoan affecting a large range of vertebrates) on both qualitative and quantitative composition of gut microbiota in a CD-1 neonatal mouse model. Methods 5-day-old neonate mice were experimentally infected with 105Cryptosporidium parvum Iowa oocysts by oesophageal gavage. The intestinal microbiota of both infected (Cp+) and uninfected (Cp−) mice groups was examined by high-throughput sequencing of the bacterial 16S rDNA gene V3–V4 hypervariable region. Results The most consistent change in the microbiota composition of Cp+ mice was the increased proportion of bacterial communities belonging to the Phylum Bacteroidetes. In contrast, the microbiota of Cp− mice was associated with increased proportions of several Firmicutes and Actinobacteria phyla members. Conclusion For the first time, our study provides evidence of an association between cryptosporidial infection and gut dysbiosis, thus contributing valuable knowledge to the as-yet little-explored field of Cryptosporidium–microbiota interactions in a neonatal mouse model.
- Published
- 2019