1. Th17 expression and IL-17 levels in laryngeal squamous cell carcinoma patients
- Author
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Fu-Jun Li, Ming Chen, Fei Yang, Zhijian Cai, and Shou-De Zhang
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Cord ,food.ingredient ,Cell ,Interleukin-23 ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,food ,Polyps ,Medicine ,Humans ,Laryngeal Neoplasms ,Aged ,Messenger RNA ,medicine.diagnostic_test ,business.industry ,Interleukin-17 ,General Medicine ,Middle Aged ,Nuclear Receptor Subfamily 1, Group F, Member 3 ,Laryngeal squamous cell carcinoma ,medicine.anatomical_structure ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Case-Control Studies ,Carcinoma, Squamous Cell ,Immunohistochemistry ,Th17 Cells ,Interleukin 17 ,business ,Polypus ,030215 immunology - Abstract
The Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging.To investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma.Eighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively.Th17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p0.01 for mRNA levels, p0.05 for protein levels).
- Published
- 2016