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A macrophage infiltrate is commonly found in enlarging subchondral cysts in osteoarthrosis (OA) and the surrounding bone. To determine whether osteoclast differentiation by these cells contributes to the increase in the number of osteoclasts and bone resorption that accompanies OA cyst enlargement, we isolated macrophages from the wall of OA cysts and co-cultured them with osteoblast-like UMR106 cells in the presence or absence of 1,25(OH)[sub 2]D[sub 3] and M-CSF. After 14 days of incubation, co-cultures of UMR106 cells and cyst-derived macrophages showed evidence of osteoclast differentiation by expression of TRAP, VNR and formation of numerous lacunar pits. We found that, unlike osteoclast precursors in monocyte and other tissue macrophage populations, the addition of M-CSF to medium is not required for osteoclast differentiation. Our findings suggest that macrophage-osteoclast differentiation is one means whereby the osteolysis associated with the enlargement of OA cysts could be effected. [ABSTRACT FROM AUTHOR]

Investigates the influence of surface finish on fluid flow along the stem-cement interface of cemented femoral components in an in vitro model. Bone cement pressurization; Contribution of capillary flow to fluid movement; Electrostatic interaction between the metal and bone cement.

Discusses the influence of the proximal femur anatomy on the formation of incomplete cement mantles in the sagittal femoral plane. Radiography of the proximal femur; Incidence of femoral osteolysis; Femoral neck retention.