1. Local partial depletion of CD11b+cells and their influence on choroidal neovascularization using the CD11b-HSVTK mouse model
- Author
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Anja-Maria Davids, Kelly R. Miller, Sabrina Dege, Antonia M. Joussen, Tobias Brockmann, Claudia Brockmann, and Norbert Kociok
- Subjects
0301 basic medicine ,Ganciclovir ,Genetically modified mouse ,genetic structures ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,medicine ,Retina ,biology ,Microglia ,Chemistry ,Retinal ,General Medicine ,eye diseases ,Ophthalmology ,030104 developmental biology ,Choroidal neovascularization ,medicine.anatomical_structure ,Integrin alpha M ,030221 ophthalmology & optometry ,biology.protein ,sense organs ,medicine.symptom ,medicine.drug - Abstract
PURPOSE To assess the influence of retinal macrophages and microglia on the formation of choroidal neovascularization (CNV). Therefore, we used a transgenic mouse (CD11b-HSVTK) in which the application of ganciclovir (GCV) results in a depletion of CD11b+ cells. METHODS We first investigated if a local depletion of CD11b+ macrophages and microglia in the retina is feasible. In a second step, the influence of CD11b+ cell depletion on CNV formation was analysed. One eye of each CD11b-HSVTK mouse was injected with GCV, and the fellow eye received sodium chloride solution (NaCl). Cell counting was performed at day 3 and 7 (one injection) or at day 14 and 21 (two injections). Choroidal neovascularization (CNV) was induced by argon laser and analysed at day 14. RESULTS The most effective CD11b+ cell depletion was achieved 7 days after a single injection and 14 days after two injections of GCV. After two injections of GCV, we found a significant reduction of CD11b+ cells in central (52 ± 23.9 cells/mm2 ) and peripheral retina (53 ± 20.6 cells/mm2 ); compared to eyes received NaCl (216 ± 49.0 and 210 ± 50.5 cells/mm2 , p
- Published
- 2018
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