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Start Over Author "R. Sipka" Journal acta microbiologica et immunologica hungarica
2 results on '"R. Sipka"'

1. Chlamydophila pneumoniaeand Human Cytomegalovirus in Atherosclerotic Carotid Plaques - Combined Presence and Possible Interactions

Author

Pál Barzó, R. Sipka, Eva Gonczol, Márta Jäckel, Valéria Endrész, Zoltan Kis, Tibor Kerényi, Katalin Burián, Dezső P. Virok, Mihály Bodosi, D. E. Nelson, and Laszlo Kari

Subjects
DNA, Bacterial, Male, Human cytomegalovirus, Population, Cytomegalovirus, Inflammation, Coronary Artery Disease, medicine.disease_cause, Polymerase Chain Reaction, Monocytes, Cell Line, Microbiology, Antigen, medicine, Humans, Carotid Stenosis, RNA, Messenger, education, Antigens, Viral, Chlamydophila Infections, Aged, Antigens, Bacterial, Endarterectomy, Carotid, education.field_of_study, General Immunology and Microbiology, biology, General Medicine, Chlamydophila pneumoniae, Middle Aged, beta-Thromboglobulin, medicine.disease, Coronary Vessels, Immunohistochemistry, Virology, Cytomegalovirus Infections, DNA, Viral, Coinfection, biology.protein, Female, medicine.symptom, Antibody
Abstract

The aim of our study was to investigate the combination of Chlamydophila pneumoniae and human cytomegalovirus (HCMV) as a pathogenic factor in atherosclerosis. Accordingly, we tested by means of PCR and immunohistochemistry the presence of these pathogens in the same atherosclerotic carotid specimen. The histology of the samples and the patients' antibodies against these pathogens were evaluated. Further, we examined the impact of C. pneumoniae and HCMV infection on the gene expression of the human monocytic cell line U937. Six of the 22 samples contained only C. pneumoniae, 4 contained only HCMV, 7 contained both C. pneumoniae DNA and/or antigens of both pathogens, and 5 samples were negative. No correlation was found between the presence of these microbes and either the cellular structure of the plaques, or the serostatus of the patients. The infection of U937 cells with HCMV and especially C. pneumoniae induced inflammation and atherosclerosis-related genes. Furthermore, the doubly-infected cells produced higher levels of the mRNA of pro-platelet basic protein and fatty acid binding protein 4. In conclusion, C. pneumoniae is often present in combination with HCMV in atherosclerotic carotid lesions. The in vitro coinfection model reveals that the doubly-infected monocytes are potent expressors of proatherosclerotic genes, suggesting that this coinfected population may accelerate the process of atherosclerosis.

Published
2006
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2. Chlamydophila pneumoniae and human cytomegalovirus in atherosclerotic carotid plaques--combined presence and possible interactions.

Author

Virok D, Kis Z, Kari L, Barzo P, Sipka R, Burian K, Nelson DE, Jackel M, Kerenyi T, Bodosi M, Gönczol E, and Endresz V

Subjects
Aged, Antigens, Bacterial isolation & purification, Antigens, Bacterial metabolism, Antigens, Viral isolation & purification, Antigens, Viral metabolism, Carotid Stenosis microbiology, Carotid Stenosis pathology, Carotid Stenosis surgery, Cell Line, Chlamydophila Infections diagnosis, Chlamydophila pneumoniae genetics, Chlamydophila pneumoniae immunology, Coronary Artery Disease microbiology, Coronary Artery Disease pathology, Coronary Artery Disease surgery, Coronary Vessels pathology, Coronary Vessels surgery, Cytomegalovirus genetics, Cytomegalovirus immunology, Cytomegalovirus Infections diagnosis, DNA, Bacterial genetics, DNA, Viral genetics, Endarterectomy, Carotid, Female, Humans, Immunohistochemistry, Male, Middle Aged, Monocytes metabolism, Monocytes microbiology, Monocytes virology, Polymerase Chain Reaction, RNA, Messenger analysis, RNA, Messenger biosynthesis, beta-Thromboglobulin genetics, beta-Thromboglobulin metabolism, Carotid Stenosis etiology, Chlamydophila Infections complications, Chlamydophila pneumoniae isolation & purification, Coronary Artery Disease etiology, Coronary Vessels microbiology, Cytomegalovirus isolation & purification, Cytomegalovirus Infections complications
Abstract

The aim of our study was to investigate the combination of Chlamydophila pneumoniae and human cytomegalovirus (HCMV) as a pathogenic factor in atherosclerosis. Accordingly, we tested by means of PCR and immunohistochemistry the presence of these pathogens in the same atherosclerotic carotid specimen. The histology of the samples and the patients' antibodies against these pathogens were evaluated. Further, we examined the impact of C. pneumoniae and HCMV infection on the gene expression of the human monocytic cell line U937. Six of the 22 samples contained only C. pneumoniae, 4 contained only HCMV, 7 contained both C. pneumoniae DNA and/or antigens of both pathogens, and 5 samples were negative. No correlation was found between the presence of these microbes and either the cellular structure of the plaques, or the serostatus of the patients. The infection of U937 cells with HCMV and especially C. pneumoniae induced inflammation and atherosclerosis-related genes. Furthermore, the doubly-infected cells produced higher levels of the mRNA of pro-platelet basic protein and fatty acid binding protein 4. In conclusion, C. pneumoniae is often present in combination with HCMV in atherosclerotic carotid lesions. The in vitro coinfection model reveals that the doubly-infected monocytes are potent expressors of proatherosclerotic genes, suggesting that this coinfected population may accelerate the process of atherosclerosis.

Published
2006
Full Text
View/download PDF

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