1. ENVIRONMENTAL IMPACT ASSESSMENT OF TRANSGENIC PLUMS ON THE DIVERSITY OF PLUM POX VIRUS POPULATIONS
- Author
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Ioana Gaboreanu, N. Capote, B. Ferencz, Luminita Zagrai, Michel Ravelonandro, Ioan Zagrai, Doru Pamfil, Octavian Popescu, Ralph Scorza, Academy for Agricultural and Forestry Sciences and Romanian Horticulture Society, Partenaires INRAE, Génomique, développement et pouvoir pathogène (GD2P), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), University of Agricultural Sciences and Veterinary Medicine, Babes-Bolyai University [Cluj-Napoca] (UBB), Appalachian Fruit Research Station, USDA-ARS : Agricultural Research Service, Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA), and ProdInra, Migration
- Subjects
0106 biological sciences ,SAFETY ISSUE ,CP GENE ,[SDV]Life Sciences [q-bio] ,Transgene ,media_common.quotation_subject ,PPV ,PLUM POX VIRUS ,030312 virology ,Horticulture ,Biology ,01 natural sciences ,03 medical and health sciences ,PATHOGEN DERIVED RESISTANCE ,RECOMBINANT VIRUSES ,Pox virus ,Environmental impact assessment ,C5 ,media_common ,2. Zero hunger ,BIOTECHNOLOGIE ,0303 health sciences ,business.industry ,POTYVIRUS ,VIROLOGIE ,Biotechnology ,[SDV] Life Sciences [q-bio] ,business ,010606 plant biology & botany ,Diversity (politics) - Abstract
International audience; Plum pox virus (PPV) is considered as the most detrimental virus of stone fruit-trees, causing serious yield losses. Transgenic plums were produced and released in field. In order to analyze the environmental effects related to the release of virus-resistant transgenic crops we studied the diversity of PPV populations in GM plum clones containing the PPV coat protein (CP) gene. We compared the serological and molecular variability of PPV detected in the transgenic trees versus those found in conventional plums. PPV strains were serologically determined by TAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies. Molecular strain typing was performed targeting three genomic regions (Cter) CP, (Cter) NIb-(Nter) CP and CI. RFLP analysis was used to distinguish the two major strains, D and M based on an Rsa I polymorphism located in (Cter)CP. PCR products spanning (Cter) CP and (Cter) NIb-(Nter) CP regions were sequenced. The results revealed that there was no significant difference between PPV isolates from transgenic and conventional plums. This study confirmed that the transgenic plums evaluated in this report do not represent an environmental risk through the production of any emerging PPV variants.
- Published
- 2008
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