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Summary Using immunohistochemical methods at both light and electron microscopical levels, we have studied epidermal growth factor in the uterine epithelium during early pregnancy and up to the time of blastocyst implantation. We report that the distribution of this growth factor changed markedly over the period of study and that it was gradually de-expressed across the entire uterine epithelium as pregnancy advanced towards the time of implantation. At the electron microscopical level, immunogold labelling clearly showed label associated with both lateral and basal plasma membranes very early in pregnancy but not by the time of implantation. We suggest that the de-expression of this molecule may indicate its role in the removal of mechanisms which inhibit uterine receptivity for implantation.

Summary In this retrospective and quantitated study on banked tissue we found that, compared to normal uterine epithelial cells, growth hormone (GH) is increased 3.4-fold in endometriosis and 3.8-fold in endometrial adenocarcinoma. Similarly, interleukin-6 (IL-6) is increased 2.4-fold in endometriosis and 4.4-fold in endometrial adenocarcinoma. These proteins appear to be involved in the progression of both these conditions. GH is particularly interesting in this context since it is known to not only promote cellular proliferation but also reduces cell–cell adhesion, thus allowing individual cells to break away from their parent architecture. Our results suggest that both IL-6 and GH may play a role in the progression of both endometriosis and endometrial carcinoma.

In the mature rat ventral prostate, epithelial proliferation is accompanied by significant upregulation of tyrosine kinase A, alpha1B and muscarinic acetylcholine M2 receptors as well as the synaptic vesicle-associated membrane proteins synaptobrevin and SV2 as compared with immature prostate tissue. The adrenergic receptors beta1, alpha2A and alpha1 were also up-regulated and translocated in mature rat prostate tissue. Expression of the Schwann cell/axonal marker S100 remained unchanged. These results are suggestive of a marked increase in metabolic activity, calcium influx and autonomic receptor expression in the aging prostate. These changes were not accompanied by an increase in the number of axons.