1. Effects of sodium valproate on the chromatin of Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae) under in vitro culture conditions
- Author
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João Aristeu da Rosa, Alessandra Bassani, Maria Luiza S. Mello, Juliana Damieli Nascimento, Vera Lúcia Cortiço Corrêa Rodrigues, Douglas S. Santos, Marina Amorim Rocha, Universidade Estadual de Campinas (UNICAMP), Superintendence for Control of Endemic Diseases (SUCEN), and Universidade Estadual Paulista (Unesp)
- Subjects
0301 basic medicine ,Malpighian tubule system ,Histology ,Euchromatin ,medicine.drug_class ,Biology ,Malpighian Tubules ,Chromatin remodeling ,03 medical and health sciences ,Histone H3 ,0302 clinical medicine ,Non-histone protein ,Heterochromatin ,Histone methylation ,medicine ,Animals ,Triatoma ,Cell Nucleus ,Histone deacetylase inhibitor ,Valproic Acid ,Acetylation ,Cell Biology ,General Medicine ,Molecular biology ,Chromatin ,Histone Deacetylase Inhibitors ,030104 developmental biology ,Histone acetylation ,030220 oncology & carcinogenesis ,lipids (amino acids, peptides, and proteins) ,Epigenetics ,Insect - Abstract
Made available in DSpace on 2021-06-25T10:22:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Sodium valproate (VPA) is a classic anticonvulsive, a histone deacetylase inhibitor, and a chromatin remodeling inducer. When injected into specimens of Triatoma infestans, a vector of Chagas disease, VPA affects the chromatin supraorganization of chromocenter heterochromatin in only a few cells of the Malpighian tubules. To test whether this result was explained by the inaccessibility of all of the organ's cells to the drug, we investigated the nuclear phenotypes and global acetylation of lysine 9 in histone H3 (H3K9ac) in Malpighian tubules cultivated in vitro for 1–24 h in the presence of 0.05 mM–1 mM VPA. The present results revealed that the chromatin decondensation event in the chromocenter body, which was detected only under low VPA concentrations up to a 4-h treatment, was not frequent during organ culture, similar to the results for injected insects. Cultivation of T. infestans Malpighian tubules in vitro for 24 h revealed inadequate for cell preservation even in the absence of the drug. Immunofluorescence signals for H3K9ac following VPA treatment showed a slightly increased intensity in the euchromatin, but were never detected in the chromocenter bodies, except with great intensity at their periphery, where the 18S rDNA is located. In conclusion, when VPA affects the chromocenter heterochromatin in this animal cell model, it occurs through a pathway that excludes a classic global H3K9ac mark. Investigation of nonhistone proteins associated with histone methylation marks is still required to further explain the differential response of T. infestans chromatin to VPA. Department of Structural and Functional Biology Institute of Biology University of Campinas (Unicamp) Superintendence for Control of Endemic Diseases (SUCEN) School of Pharmaceutical Sciences São Paulo State University (Unesp) School of Pharmaceutical Sciences São Paulo State University (Unesp) CNPq: 304797/2019-7 CNPq: 307398/2018-8
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- 2020