1. Diffuse Large B Cell Lymphoma without Immunoglobulin Light Chain Restriction by Flow Cytometry
- Author
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Naoto Tomita, Hiroyuki Fujita, Koji Ogawa, Jun Taguchi, Shigeki Motomura, Sachiya Takemura, Chizuko Hashimoto, Rie Hyo, Yoshiaki Ishigatsubo, Shin Fujisawa, and Kengo Takeuchi
- Subjects
Adult ,Male ,Genes, myc ,Chromosomal translocation ,Biology ,CD5 Antigens ,urologic and male genital diseases ,Immunoglobulin light chain ,Translocation, Genetic ,Flow cytometry ,Immunoglobulin kappa-Chains ,Immunoglobulin lambda-Chains ,Antigen ,Antigens, Neoplasm ,medicine ,Humans ,B cell ,Aged ,Retrospective Studies ,B-Lymphocytes ,medicine.diagnostic_test ,Hematology ,General Medicine ,Middle Aged ,Flow Cytometry ,Prognosis ,bacterial infections and mycoses ,medicine.disease ,Survival Analysis ,Molecular biology ,female genital diseases and pregnancy complications ,Clone Cells ,Genes, bcl-2 ,Lymphoma ,DNA-Binding Proteins ,medicine.anatomical_structure ,embryonic structures ,Proto-Oncogene Proteins c-bcl-6 ,Female ,Immunoglobulin Light Chains ,Neprilysin ,Lymphoma, Large B-Cell, Diffuse ,CD5 ,human activities ,Diffuse large B-cell lymphoma - Abstract
Background: The existence of immunoglobulin light chain restriction (LCR) strongly indicates B cell monoclonality. Although LCR-deficient B cell malignancies are often observed, their details are barely known. Methods: We retrospectively analyzed LCR-negative diffuse large B cell lymphoma (DLBCL) to elucidate their clinical features. Consecutive DLBCL patients (n = 119), whose histological diagnostic specimens were analyzed by flow cytometry (FCM), were divided into 2 groups: LCR-positive and LCR-negative DLBCL. Cases wherein FCM did not capture tumor cells were excluded. Results: There were 91 LCR-positive (76%) and 28 LCR-negative (24%) DLBCL. The 2 groups did not differ with regard to background, including the International Prognostic Index (IPI), each factor of IPI, gender, bulky mass and B-symptoms. FCM analysis showed that CD10-positive cases were less frequent in the LCR-negative DLBCL group than in the LCR-positive DLBCL group. CD5-positive cases were absent in the LCR-negative DLBCL group. Chromosomal analysis showed that the frequency of BCL2, BCL6 and MYC translocations did not differ between the groups. There was no survival difference in the groups. Conclusion: LCR-negative DLBCL accounts for about one fourth of all DLBCL and their prognosis is similar to that of LCR-positive DLBCL. CD10-negative status might characterize LCR-negative DLBCL.
- Published
- 2009
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