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This paper addresses a soil bio-stabilization technique using bacterial enzyme-induced calcium carbonate precipitation (BEICP) as an alternative to previous conventional methods including microbial-induced carbonate precipitation and plant-derived enzyme-induced carbonate precipitation. The extracted urease enzyme of viable S. pasteurii was used as a biological source along with calcium chloride and urea to solidify sandy soil and silty sand soil. The bio-treated soil columns were subjected to freeze and thaw (F–T) cycling for a durability evaluation. Engineering properties of bio-cemented soil including unconfined compressive strength, calcium carbonate contents, moisture contents, porosity, permeability, and microstructure were examined before and after the F–T durations. It was found that although bio-stabilizer was able to increase a frost duration of soil, the F–T cycling significantly impacted on the compressive strength of bio-treated soil, due to a formation of microcracks. This investigation has revealed that the BEICP method provided a similar capacity in F–T resistance of soil as using the traditional Portland cement stabilizer, whereas the class F fly ash did not improve F–T durability of medium dense soil. [ABSTRACT FROM AUTHOR]