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Your search keyword '"Valeria Menchise"' showing total 2 results
Start Over Author "Valeria Menchise" Journal acta crystallographica. section d, biological crystallography
2 results on '"Valeria Menchise"'

1. Crystallization and preliminary X-ray diffraction studies of Aes acetyl-esterase from Escherichia coli

Author

Valeria Menchise, Giuseppe Manco, Giuseppina De Simone, Luigi Mandrich, Nicola Sorrentino, Carlo Pedone, and Mosè Rossi

Subjects
Protein Conformation, chemistry.chemical_element, Polyethylene glycol, Crystallography, X-Ray, Esterase, law.invention, chemistry.chemical_compound, Structural Biology, law, Escherichia coli, Crystallization, Lipase, biology, Magnesium, Escherichia coli Proteins, Resolution (electron density), Proteins, General Medicine, Recombinant Proteins, Crystallography, chemistry, X-ray crystallography, biology.protein, Orthorhombic crystal system, Acetylesterase, Synchrotrons
Abstract

The acetyl-esterase Aes from Escherichia coli, which belongs to the HSL group of the esterase/lipase superfamily, has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 8000 as a precipitant and magnesium chloride as an additive. Crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 110.0, b = 190.6, c = 218.6 A. A complete data set has been collected to 2.5 A resolution at the Elettra synchrotron source, Trieste using a single frozen crystal. Packing density considerations agree with 10-16 monomers in the asymmetric unit, with a corresponding solvent content of 61-38%.

Published
2003

2. Crystallization and preliminary X-ray diffraction studies of a D-lysine-based chiral PNA-DNA duplex

Author

Nicola Sorrentino, Carlo Pedone, Roberto Corradini, Giuseppina De Simone, Michele Saviano, Valeria Menchise, Stefano Sforza, and Alessandra Romanelli

Subjects
Peptide Nucleic Acids, Protein Conformation, Lysine, General Medicine, DNA, Crystallography, X-Ray, Synchrotron, law.invention, Polyethylene Glycols, Crystal, chemistry.chemical_compound, Crystallography, Monomer, chemistry, Structural Biology, law, Duplex (building), X-ray crystallography, PEG ratio, Nucleic Acid Conformation, Crystallization, Chirality (chemistry)
Abstract

A 10-mer duplex formed between a PNA containing a 'chiral box' of three adjacent D-Lys-based monomers and its complementary DNA strand has been crystallized for the first time. Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer. The crystals belong to the space group P3(1) or to its enantiomorph P3(2), with unit-cell parameters a = b = 35.00, c = 35.91 A. A complete data set has been collected at the synchrotron source Elettra in Trieste to 1.85 A resolution, using a single frozen crystal.

Published
2001

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