Search

Your search keyword '"Wen, Jinjin"' showing total 2 results
Start Over Author "Wen, Jinjin" Journal acta crystallographica section f structural biology and crystallization communications
2 results on '"Wen, Jinjin"'

2. Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.

Author

Wen J, Li Z, He G, Xu S, Zhao B, Zhu X, Dong H, and Ju J

Subjects
Amino Acid Sequence, Crystallization, Crystallography, X-Ray, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Sequence Alignment, Alanine Dehydrogenase chemistry, Bacillus enzymology, Bacterial Proteins chemistry
Abstract

Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M Tris-HCl pH 8.0, 0.2 M LiSO4, 22%(w/v) PEG 3350. X-ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°.

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results