1. Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells.
- Author
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Speroni L, Gasparri J, de los A Bustuoabad V, Chiaramoni NS, Smagur A, Szala S, Taira MC, and del V Alonso S
- Subjects
- Amines, Analysis of Variance, Animals, Cell Line, Tumor, Cell Survival, Disease Progression, Fatty Acids, Monounsaturated, Genes, Reporter, Interleukin-12 metabolism, Liposomes, Melanoma, Experimental metabolism, Melanoma, Experimental mortality, Melanoma, Experimental pathology, Mice, Mice, Inbred C57BL, Neoplasm Transplantation, Quaternary Ammonium Compounds, Tumor Burden, Interleukin-12 genetics, Melanoma, Experimental therapy, Transfection methods
- Abstract
Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
- Published
- 2009