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In this study, it was aimed to determine the benthic macroinvertebrate fauna of Demre Stream in Antalya. Benthic macroinvertebrate samples were taken seasonally from 12 stations determined on Demre Stream between April 2015 and December 2015 and the obtained individuals belonging to Clitellata, Rhabditophora, Gastropoda, Insecta, and Arachnida groups Macroinvertebrate based clustering of stations was calculated by using UPGMA analysis. The lowest similarity was determined between the 3rd and 10th stations, and the highest similarity was between the 8th and 11th stations. Simpson and Shannon-Wiener diversity indices were applied to determine the diversity values of the stations. According to both diversity indices, the highest diversity value was reached at the 5th station, while the lowest diversity value was reached at the 3rd station. This study is the first study to determine the benthic fauna of the Demre Stream and therefore all the groups identified are the first records for the Demre Stream. [ABSTRACT FROM AUTHOR]